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pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

Plasmid

#174026

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-QF2

Use

Zebrafish knock-in tagging

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2039

Plasmid

#139929

Purpose

Complementation plasmid for LetAB without LetB TM region

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(43-877)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57A

Plasmid

#169927

Purpose

WT U6 with a U57A mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57A

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKP381

Plasmid

#167592

Purpose

Express TonB H20A protein

Depositor

Kathleen Postle

Article

Larsen et al J Bacteriol. 2007 Apr;189(7):2825-33. do

Insert

tonB H20A

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSWD95

Plasmid

#158617

Purpose

CckA FRET Sensor

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nls

Plasmid

#108882

Purpose

Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls

Use

Multisite gateway

Available Since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1889

Plasmid

#139893

Purpose

Complementation plasmid for LetAB with LetB PLL4 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta459-360)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1996

Plasmid

#139900

Purpose

Expression plasmid for LetB PLL4 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta459-360)-6xHis

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2041

Plasmid

#139926

Purpose

Complementation plasmid for LetAB with 4 ring (rings 1237) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB (1-391; 747-877)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1892

Plasmid

#139896

Purpose

Complementation plasmid for LetAB with LetB PLL7 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta816-826, S815G, T827G)

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2040

Plasmid

#139930

Purpose

Complementation plasmid for LetAB with LetB TM region replaced by PelB leader sequence

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-(PelB-LetB(43-877))-6xHis

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2071

Plasmid

#139928

Purpose

Complementation plasmid for LetAB operon with LetA and LetB genes deoverlapped

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB_deoverlapped

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1859

Plasmid

#139906

Purpose

Complementation plasmid for LetAB with LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(V247N)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1858

Plasmid

#139905

Purpose

Complementation plasmid for LetAB with LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L246N)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1890

Plasmid

#139894

Purpose

Complementation plasmid for LetAB with LetB PLL5 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta581-590, K580G, Q591G)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2044

Plasmid

#139923

Purpose

Expression plasmid for 4 ring (rings 1237) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB (43-391; 747-877)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1731

Plasmid

#139922

Purpose

Expression plasmid for LetB mutant E854Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; E854Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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