We narrowed to 1,853 results for: FAST
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Plasmid#119830PurposeProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluh
TagsGSTExpressionBacterialMutationE25APromoterT7Available sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p130Cas Y15F
Plasmid#186154PurposeFor expressing recombinant p130Cas in insect cells.DepositorInsertBCAR1 (Bcar1 Rat)
TagsHis6 (Tev)ExpressionInsectMutation15Y residues mutated to AlaninePromoterpolyhedrinAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
TagsGSTExpressionBacterialMutationT92APromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFB-EPCP5
Plasmid#228879PurposeExpression of Z-tagged LRRK2 KW using BaculovirusesDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
TagsHis-Z-TEVExpressionInsectMutationAmino acids deleted 1-1846Available sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFBOH-LIC_DDB1:1-1140
Plasmid#210886PurposeInsect expression of full-length human DDB1. Untagged.DepositorAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_DCAF11:1-546
Plasmid#210907PurposeInsect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.DepositorInsertDCAF11:M1-Q546 (DCAF11 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationR207H natural variant (VAR_020121)PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIG-832_NY-ESO-1_TCR_FAS/4-1BB
Plasmid#207495PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFAS/4-1BB, NY-ESO-1_TCR (FAS Human)
UseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-836_NY-ESO-1_TCR_FAS/OX40
Plasmid#207499PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFAS/OX40, NY-ESO-1_TCR (FAS Human)
UseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD-MyoD
Plasmid#25992DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyoD-MyoD homodimer (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_EIF3B:127-814
Plasmid#210909PurposeInsect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF3B:D127-E814 (EIF3B Human)
TagsMHHHHHHSSGRENLYFQGMQExpressionInsectMutationY760C sequence conflictPromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
ExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
ExpressionMammalianPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pD649-HAsp-FAS-Fc(DAPA)-AviTag-6xHis
Plasmid#156541PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertFAS (FAS Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAL3438
Plasmid#47157DepositorInsertZebrafishCommunity-fastkd3-left (im:7147223 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianPromoterCMVAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pECNUS4_MP
Plasmid#228358PurposeABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screeningDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRPS5AAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS3
Plasmid#184886PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LHP1049 - pFB-YCK2
Plasmid#32433DepositorAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pECNUS2
Plasmid#184885PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRPS5AAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only