We narrowed to 1,830 results for: FAST

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QPRTA-c002

Plasmid

#205115

Purpose

QPRT protein expression

Depositor

Paul Brennan

Insert

QPRT (QPRT Human)

Tags

TEV-6His

Expression

Insect

Mutation

WT

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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RABEP1A-c019

Plasmid

#175477

Purpose

Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

Depositor

Opher Gileadi

Insert

RABEP1 (RABEP1 Human)

Tags

TEV-TwinStrep

Expression

Insect

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEND-192_Cacnes_HS

Plasmid

#225621

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

Depositor

Marc Güell

Publication

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-193_Cacnes_HS

Plasmid

#225622

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

Depositor

Marc Güell

Publication

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TMPRSS3:72-454_CO

Plasmid

#220880

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

TMPRSS3 (TMPRSS3 Human)

Use

Baculovirus expresssion

Tags

8xHis and melittin signal sequence

Promoter

Polyhedrin

Available since

Sept. 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LHP1049 - pFB-YCK2

Plasmid

#32433

Depositor

Linda Hicke

Insert

YCK2 (YCK2 Budding Yeast)

Expression

Insect

Mutation

Y193L

Promoter

P-P10 and P-Pol

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO

Plasmid

#192382

Purpose

To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBNJ26.3

Plasmid

#66876

Purpose

For CFP tagging via transposon insertion.

Depositor

Thomas Hughes

Publication

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eCFP in a transposon

Use

Source for a tn7 transposon

Tags

eCFP

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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BDH2_FL_BioN

Plasmid

#195870

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BDH2 (BDH2 Human)

Use

Pfbd-bira, baculovirus expression

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Promoter

Polyhedrin

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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S

Plasmid

#167172

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

Depositor

Susanne Dobler

Publication

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2 HRD

Plasmid

#207544

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human WIPI2 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFBMBD4-C-GFP (pc1567)

Plasmid

#211714

Purpose

Expresses fl. MBD4 GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD4

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_WDR54:1-334

Plasmid

#210931

Purpose

Insect expression of full-length human WDR54. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR54:M1-V334 (WDR54 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CORO1C:1-474

Plasmid

#210920

Purpose

Insect expression of full-length human CORO1C. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1C:M1-A474 (CORO1C Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_DDA1:1-102

Plasmid

#210902

Purpose

Insect expression of full-length human DDA1. Untagged.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDA1:M1-T102 (DDA1 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pIG-837_NY-ESO-1_TCR_tFAS

Plasmid

#207500

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Publication

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tFAS, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FAK

Plasmid

#186141

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Publication

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH2_G674A

Plasmid

#134874

Purpose

Expression human MSH2 G674 mutant protein using baculovirus

Depositor

Peggy Hsieh

Publication

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH2_G674A (MSH2 Human)

Expression

Insect

Mutation

Changed Glycine to Alanine at aa#674

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(K14A)

Plasmid

#86562

Purpose

Expresses human full-length Akt1(K14A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

K14A mutation in the PH domain

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_SMU1:1-513

Plasmid

#210877

Purpose

Insect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMU1:M1-P513 (SMU1 Human)

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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