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Showing: 1561 - 1580 of 2760 results

  1. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

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  5. pHAGE-GFP

    Plasmid
    #106281
    Purpose
    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106281-plasmid-map-sequence-id-212556

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  12. pDIRECT_10E

    Plasmid
    #91209
    Purpose
    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91209-plasmid-map-sequence-id-182248

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  14. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

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  16. piggyFlex

    Plasmid
    #218234
    Purpose
    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
    Depositor
    Jay Shendure
    Article
    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    218234-plasmid-map-sequence-id-432525

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  19. pMOD_B2520

    Plasmid
    #91077
    Purpose
    Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91077-plasmid-map-sequence-id-181905

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Showing: 1561 - 1580 of 2760 results