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Plasmid#118180PurposeCRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide3
Plasmid#118183PurposeCRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide4
Plasmid#118176PurposeCRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide4
Plasmid#118172PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1
Plasmid#118177PurposeCRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP
Plasmid#174876PurposePiggyBac transposon vector for expression of RNAPII Ser2ph mintbodyDepositorInsertRNAPII Ser2ph mintbody-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-44B12mut23(Ser5ph)-SNAP
Plasmid#174877PurposePiggyBac transposon vector for expression of RNAPII Ser5ph mintbodyDepositorInsertRNAPII Ser5ph mintbody-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR
Plasmid#174883PurposeTargeting vector for generating Pou5f1 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Pou5f1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSI2-DCD
Plasmid#166968PurposeExpress the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADARDepositorInsertMSI2-DCD
UseRetroviralTagsMSI2-DCDExpressionMammalianMutationN495SPromoterMSCVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-His-GvpC-ssrA
Plasmid#153297PurposeExpresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertGas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
UseTagsHis-tag and ssrA tagExpressionBacterialMutation1) N-terminal 6xHis tag flanked by 1x glycine res…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl K
Plasmid#89380PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl F
Plasmid#89375PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl G
Plasmid#89376PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl J
Plasmid#89379PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl E
Plasmid#89374PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl I
Plasmid#89378PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl H
Plasmid#89377PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
NHA-Delta 10
Plasmid#36925DepositorInsertTNGW1
UseTagsNHAExpressionMammalianMutationaa 566-1343 onlyPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only