We narrowed to 10,315 results for: plasmids 101

1. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169591

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin-2

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS1026-Tier1-PhCMV-3xNLS-iRFP670

   Plasmid

   #169532

   Purpose

   Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized far-red fluorescent protein iRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2381-Tier1-PhCMV-Igk_TM

   Plasmid

   #169487

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven membrane localization
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-OTp-hM3Dq-Myc

   Plasmid

   #184753

   Purpose

   To drive hM3D fused with Myc tag under the control of mouse oxytocin promoter

   Depositor

   Kazunari Miyamichi

   Article

   Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

   Insert

   hM3D (Gq)
   

   Use

   AAV

   Tags

   3xMyc

   Expression

   Mutation

   Promoter

   mouse Oxytocin promoter

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-RAP1

   Plasmid

   #84525

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   none

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

   Plasmid

   #132684

   Purpose

   AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Peptidase M20 domain containing 2 (Pm20d2 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-rap1-K651R

   Plasmid

   #84528

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K651R

   Promoter

   none

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169592

   Purpose

   Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   phloretin-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TtgO2-PCMVmin-2

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

   Plasmid

   #169589

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin-2

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid

    #169530

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

    Plasmid

    #169590

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2392-Tier1-PhCMV-p2a_medFT

    Plasmid

    #169529

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169667

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    VanO2-PCMVmin

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2406-Tier1-PhCMV-NTEV

    Plasmid

    #169501

    Purpose

    Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven N-terminal fragment of split tobacco etch virus protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-null (BPK1077)

    Plasmid

    #171128

    Purpose

    Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV and T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJS263

    Plasmid

    #134141

    Purpose

    Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    Ska1 MTBD-His (Y106G6H.15 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)/AAA-nLuc-3XFLAG

    Plasmid

    #127306

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/GGG-nLuc-3XFLAG

    Plasmid

    #127307

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 mir-1-1 reporter mir50-7

    Plasmid

    #46716

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-7
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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