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pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

Plasmid

#169337

Purpose

Expression of the LT-Fucci(SCA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SCA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TARDBP_Halo_N_allele

Plasmid

#178131

Purpose

Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TARDBP Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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VAPB_Halo_C_allele

Plasmid

#178174

Purpose

Donor vector for endogenous tagging of human VAPB at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (VAPB Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET.HaloTag.v7

Plasmid

#226197

Purpose

bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Insert

HaloTag.v7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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ITPR1_Halo_N_allele

Plasmid

#178161

Purpose

Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ITPR1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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RAB_Halo_C_allele

Plasmid

#178124

Purpose

Donor vector for endogenous tagging of human RAB at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (AGFG1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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LRRK2_Halo_N_allele

Plasmid

#178142

Purpose

Donor vector for endogenous tagging of human LRRK2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (LRRK2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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APP_Halo_C_allele

Plasmid

#178137

Purpose

Donor vector for endogenous tagging of human APP at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (APP Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_v2

Plasmid

#183692

Purpose

Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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