Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,376 results for: pET28

Showing: 351 - 400 of 2376 results

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pET28:LanII-2E

Plasmid

#225072

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2E)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-23

Plasmid

#225067

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-23)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2D

Plasmid

#225071

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2D)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AzaM

Plasmid

#224428

Purpose

Express AzaM in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaM

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AzaC

Plasmid

#224424

Purpose

Express AzaC in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaC

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AzaD

Plasmid

#224425

Purpose

Express AzaD in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaD

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AzaB

Plasmid

#224423

Purpose

Express AzaB in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaB

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AzaG

Plasmid

#224427

Purpose

Express AzaG in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaG

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AzaE

Plasmid

#224426

Purpose

Express AzaE in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaE

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pet28a+_Yjjg

Plasmid

#220228

Purpose

Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar

Depositor

Bernd Nidetzky

Insert

Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)

Tags

Histag

Expression

Bacterial

Promoter

T7

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LflChmG

Plasmid

#221343

Purpose

Express LflChmG in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmG

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-LflChmH

Plasmid

#221344

Purpose

Express LflChmH in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmH

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmF

Plasmid

#221345

Purpose

Express ChmF in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmF

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmK

Plasmid

#221346

Purpose

Express ChmK in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmK

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmL

Plasmid

#221347

Purpose

Express ChmL in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmL

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmJ

Plasmid

#221342

Purpose

Express ChmJ in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmJ

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-CdRdhA

Plasmid

#217824

Purpose

Expresses N-His-CdRdhA in E. coli

Depositor

Pamela Silver

Article

Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

Insert

N-His-CdRhdA

Tags

His6x

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-DhFld

Plasmid

#217821

Purpose

Expresses N-His-DhFld in E. coli

Depositor

Pamela Silver

Article

Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

Insert

DhFld

Tags

His6x

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_Caspase-4_W267N

Plasmid

#214314

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

W267N

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_Caspase-4_R269D

Plasmid

#214313

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

R269D

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_lemur

Plasmid

#214332

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_sheep

Plasmid

#214335

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rat

Plasmid

#214333

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Rat)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rabbit

Plasmid

#214334

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_dog

Plasmid

#214336

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-DONSON-WM

Plasmid

#208798

Purpose

Xenopus laevis His6-Myc-DONSON (W234L, M463T)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

W234L; M463T

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-DONSON-3A

Plasmid

#208800

Purpose

Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

V473A; R476A; Y481A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l3-A'

Plasmid

#209118

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l3-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l3-A'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l2-G'

Plasmid

#209122

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-G'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l2-B'

Plasmid

#209121

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-B'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l1-A'

Plasmid

#209116

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l1-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l1-A'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-G'-l2-G'

Plasmid

#209120

Purpose

Bacterial expression of CC-GEMS ligand SUMO-G'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-G'-l2-G'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l4-G

Plasmid

#209123

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l2-A'

Plasmid

#209117

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pet28b_His_RAD23A-UBA1

Plasmid

#201549

Purpose

Expresses UBA1 of human RAD23A in E. coli

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

6x His

Expression

Bacterial

Mutation

Expresses amino acids 160-205 of DNA polymerase i…

Promoter

T7

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 bleach.2

Plasmid

#191878

Purpose

Encodes a GFP design, bleach.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.2

Tags

His Tag

Expression

Bacterial

Mutation

V61L T65S V68A S72A V112I Y145M

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 mid.8

Plasmid

#191902

Purpose

Encodes a GFP design, mid.8

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.8

Tags

His Tag

Expression

Bacterial

Mutation

V61A S72T Y145M L220V

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 sf:bleach.1

Plasmid

#191929

Purpose

Encodes a GFP design, sf:bleach.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.1

Tags

His Tag

Expression

Bacterial

Mutation

T108L Y145M T167V L220V

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 mid.13

Plasmid

#191915

Purpose

Encodes a GFP design, mid.13

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.13

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 mid.6

Plasmid

#191899

Purpose

Encodes a GFP design, mid.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.6

Tags

His Tag

Expression

Bacterial

Mutation

V68M S72T Y145M V224I

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 mid.5

Plasmid

#191896

Purpose

Encodes a GFP design, mid.5

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.5

Tags

His Tag

Expression

Bacterial

Mutation

Y145M T167V H181Y L220V V224I

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 mid.4

Plasmid

#191895

Purpose

Encodes a GFP design, mid.4

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.4

Tags

His Tag

Expression

Bacterial

Mutation

V68A S72A Y145M L220V

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 sf:bleach.3

Plasmid

#191925

Purpose

Encodes a GFP design, sf:bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 bleach.3

Plasmid

#191914

Purpose

Encodes a GFP design, bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 mid.12

Plasmid

#191913

Purpose

Encodes a GFP design, mid.12

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.12

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72V Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 acid.5

Plasmid

#191903

Purpose

Encodes a GFP design, acid.5

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.5

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145M T167V H181V

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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