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Plasmid#1410DepositorUseTagsMycExpressionMammalianMutationPromoterSRalpha promoterAvailable sinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70
Plasmid#62951PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70
Plasmid#62953PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB5
Plasmid#63106PurposeExpression of HSPB5 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb5 (CRYAB Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70
Plasmid#62952PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70
Plasmid#78304PurposeCreating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-nlsLexA::GADfluw-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 1.5kb promoter/AcGFP
Plasmid#98104PurposeMouse aB crystallin 1.5kb promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin 1.5 kb promoter fragment (-150…Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 250bp promoter/AcGFP
Plasmid#98102PurposeMouse aB crystallin 250bp promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin promoter 250 bp fragment (-259…Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 800bp promoter/AcGFP
Plasmid#98103PurposeMouse aB crystallin 800bp promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin promoter 800 bp fragment (-833…Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ1228
Plasmid#11410DepositorInserteukaryotic translation initiation factor
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pScaf-10080.1
Plasmid#111410PurposepScaf phagemid with insert for generating DNA origami scaffold 10080 bases in lengthDepositorInsertpScaf-ACKN
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-uORF1,2-MET
Plasmid#214106PurposeMET 5'UTR upstream of mGFP (uORF1 and uORF2 mutated)DepositorInsertMET 5'UTR uORF1 and 2 mutated
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_5'UTR_MET_GFP
Plasmid#214103PurposeMET 5'UTR upstream of mGFPDepositorInsertMET 5'UTR
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-uORF2-MET
Plasmid#214105PurposeMET 5'UTR upstream of mGFP (uORF2 mutated)DepositorInsertMET 5'UTR uORF2 mutated
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-uORF1-MET
Plasmid#214104PurposeMET 5'UTR upstream of mGFP (uORF1 mutated)DepositorInsertMET 5'UTR
UseLentiviralTagsGFPExpressionMammalianMutationuORF1 mutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114105PurposeCre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-FIP200-MBP
Plasmid#171410PurposeFor the purifcation of human ULK1 complexDepositorInsertFIP200 (RB1CC1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA19_sgRNA-C0_M13ps
Plasmid#231410PurposeM13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)DepositorInsertsgRNA-C0
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only