We narrowed to 216 results for: 48138

1. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01

   Plasmid

   #211890

   Purpose

   Bacterial expression of CENP-T[1-242/3D]-I3-01

   Depositor

   Iain Cheeseman

   Insert

   CENP-T[1-242/3D]-I3-01 (CENPT Human, Synthetic)
   

   Expression

   Bacterial

   Mutation

   T11D, T27D, T85D

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor

   Plasmid

   #211867

   Purpose

   CENP-T(1-242) transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   CENP-T(1-242) (CENPT Human, Synthetic)
   

   Use

   CRISPR

   Mutation

   N/A

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKG113

   Plasmid

   #63073

   Purpose

   Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   CENP-T (CENPT Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Mutation

   E. coli codon optimized. G217R mutation compared …

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor

   Plasmid

   #211866

   Purpose

   CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   CENP-T(1-242)-I3-01 (CENPT Human, Synthetic)
   

   Use

   CRISPR

   Mutation

   N/A

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ZJOM31

   Plasmid

   #133645

   Purpose

   Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-TGL3-GFP (TGL3 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ZJOM90

   Plasmid

   #133647

   Purpose

   Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-mCherry-HDEL
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZJOM160

   Plasmid

   #133670

   Purpose

   Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-mTagBFP2-SKL
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ZJOM151

   Plasmid

   #133643

   Purpose

   Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-COX4-GFP (COX4 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ZJOM27

   Plasmid

   #133641

   Purpose

   Integration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pCUP1-GFP-Pho8 (PHO8 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ZJOM18

    Plasmid

    #133631

    Purpose

    Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-GFP (NAB2 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ZJOM153

    Plasmid

    #133642

    Purpose

    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    Vph1-2GFP (VPH1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor

    Plasmid

    #211888

    Purpose

    CENP-T[1-242] transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242] (CENPT Human, Synthetic)
    

    Use

    CRISPR

    Mutation

    T11A, T85A

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZJOM144

    Plasmid

    #133630

    Purpose

    Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-GFP-HDEL
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZJOM65

    Plasmid

    #133654

    Purpose

    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ZJOM68

    Plasmid

    #133657

    Purpose

    Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-TGL3-mCherry (TGL3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZJOM149

    Plasmid

    #133646

    Purpose

    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM82

    Plasmid

    #133648

    Purpose

    Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-mCherry (NAB2 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ZJOM64

    Plasmid

    #133655

    Purpose

    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-DuDre (COX4 Budding Yeast)
    

    Tags

    DuDre

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ZJOM135

    Plasmid

    #133659

    Purpose

    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZJOM143

    Plasmid

    #133667

    Purpose

    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge