Skip to main content
Addgene
Showing: 141 - 160 of 699 results

  1. pcDNA3-Cdc45-mCer

    Plasmid
    #211389
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211389-plasmid-map-sequence-id-420426

    Enlarge

  2. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid
    #112491
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S320A
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112491-plasmid-map-sequence-id-214534

    Enlarge

  3. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid
    #112489
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K377R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112489-plasmid-map-sequence-id-214536

    Enlarge

  4. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid
    #112490
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K599R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112490-plasmid-map-sequence-id-214537

    Enlarge

  5. pcDNA3.1-hRIPK1-Flag-K115R

    Plasmid
    #112488
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K115R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112488-plasmid-map-sequence-id-214538

    Enlarge

  7. pcDNA3.1_hMed25

    Plasmid
    #206958
    Purpose
    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206958-plasmid-map-sequence-id-422373

    Enlarge

  8. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid
    #189562
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189562-plasmid-map-sequence-id-374127

    Enlarge

  10. pcDNA3.1-hRIPK1-Flag-delN

    Plasmid
    #112492
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of N terminus (AA1-274 deleted)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112492-plasmid-map-sequence-id-214533

    Enlarge

  11. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid
    #112493
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of C terminus (AA290-671 deleted)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112493-plasmid-map-sequence-id-214530

    Enlarge

  12. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid
    #112494
    Purpose
    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of DD domain (AA584-671 deleted)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112494-plasmid-map-sequence-id-214528

    Enlarge

  13. pcDNA3-C17orf53_1-87-mCer

    Plasmid
    #211441
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211441-plasmid-map-sequence-id-419424

    Enlarge

  14. pcDNA3-C17orf53_1-87-mVen

    Plasmid
    #211442
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211442-plasmid-map-sequence-id-419261

    Enlarge

  16. pcDNA3-Flag-RNF125 Ring Mutant

    Plasmid
    #122046
    Purpose
    express RNF125 RM
    Depositor
    Ze'ev Ronai
    Insert
    RNF125 RM (RNF125 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ring mutant C37A, C40A
    Promoter
    Available since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122046-plasmid-map-sequence-id-242459

    Enlarge

  17. pcDNA3-sACE2v1

    Plasmid
    #145148
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145148-plasmid-map-sequence-id-292584

    Enlarge

Showing: 141 - 160 of 699 results