-
Plasmid#30015DepositorInsertpLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2307 (=HL 716 + pHL 777)
Plasmid#30021DepositorInsertpLtetO-1:MicC, pLtetO-1:RhyB, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3723 (=HL 716 + pHL 489)
Plasmid#30085DepositorInsertpLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3724 (=HL 716 + pHL 991)
Plasmid#30086DepositorInsertpLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3859 (=HL 716 + pHL 1305)
Plasmid#30089DepositorInsertpLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3450 (=HL 3262 + pHL 1191)
Plasmid#30080DepositorInsertpLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3449 (=HL 3262 + pHL 1179)
Plasmid#30079DepositorInsertpLtetO-1:mCherry, pLlacO-1:fhlA::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2304 (=HL 716 + pHL 269)
Plasmid#30019DepositorInsertpLtetO-1:MicC-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2300 (=HL 716 + pHL 745)
Plasmid#30017DepositorInsertpLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 1178 (=HL 862 + pHL 282)
Plasmid#30012DepositorInsertpLtetO-1:mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2314 (=HL 716 + pHL 818)
Plasmid#30025DepositorInsertpLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2625 (=HL 716 + pHL 908)
Plasmid#30028DepositorInsertpLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3612 (=HL 716 + pHL 841)
Plasmid#30082DepositorInsertpLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3619 (=HL 716 + pHL 1228)
Plasmid#30083DepositorInsertpLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL7 SMR1791
Plasmid#28030DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex VP16 (F442A) HA (P#1711)
Plasmid#14596DepositorInsertLexA
UseTagsHA, Ubiquitin, and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOP1
Plasmid#112595PurposeUsed for expression of protein in E. coli as non-fusionsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mcherry1
Plasmid#87144PurposeConstitutive E. coli mcherry ExpressionDepositorInsertmcherry
UseTagsExpressionBacterialMutationPromoterConstitutive proDAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLS.402
Plasmid#187202PurposeExpresses retron Eco1 RTDepositorInsertRetron Eco1 Reverse Transcriptase
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermphRAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen
Plasmid#182387PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen fusionDepositorInsertVNp-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LM627
Plasmid#191551PurposeLM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-hisDepositorInsertccdb
UseTagsSNAC-tag, HIS-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-L11
Plasmid#127831PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertlacI (lacI Escherichia coli)
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(WT).J23115-gfp
Plasmid#197872PurposeArabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expressionDepositorInsertspart kilA/repL
gfp
UseTagsExpressionBacterialMutationcontains only the 24 bp of 3'-end of kilA ge…PromoterBba_J23115 and PBADAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
UseTagsExpressionBacterialMutationPromoterArabinose-inducible pBADAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-DsbC
Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
eBFP2 (eBFP2)_CD
Plasmid#66034PurposeMoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]DepositorInsertFluorescent reporter - BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-YFP
Plasmid#186563Purposep15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsNoneExpressionBacterial and MammalianMutationNonePromoterPromotorlessAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
UseTagsExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB
Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP568
Plasmid#222725PurposepNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-mNeongreen
Plasmid#182388PurposeBacterial expression of Vesicle Nucleating peptide6-mNeongreen fusionDepositorInsertVNp6-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPExpressionMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…PromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15ABAD- YFP
Plasmid#186571Purposep15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOZITX-S1
Plasmid#184925PurposeExpresses the catalytic S1 subunit of OZITX (EcPltAB)DepositorInsertcodon optimized S1 subunit of OZITX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_116_LVL2 cam
Plasmid#179332PurposeNT-CRISPR plasmid for a single gRNA.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Traptavidin-E6
Plasmid#59549PurposepET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatographyDepositorInsertCore Traptavidin-E6
UseTagsE6 tagExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBMN DHFR(DD)-YFP
Plasmid#29325DepositorInsertDHFR(DD)-YFP
UseRetroviralTagsHA and YFPExpressionMutationChange Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …PromoterAvailable sinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-His6
Plasmid#182386PurposeBacterial expression of Vesicle Nucleating peptideDepositorInsertVNp-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP2270
Plasmid#222733PurposepNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (lacZ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p15aC-4D1D-5
Plasmid#107866PurposeBacterial expression of PIS, PI4K and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-trc- YFP
Plasmid#186569Purposep15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.400
Plasmid#187201PurposeExpresses retron Eco4 RTDepositorInsertRetron Eco4 Reverse Transcriptase
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermphRAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-DARPinOFF7
Plasmid#182398PurposeBacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusionDepositorInsertVNp6-DARPinOFF7
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPN-mKate (PT7LacO-PN-mKate)
Plasmid#191126PurposeExpression of protein N tagged with mKate from a T7-lac inducible promoterDepositorInsertProtein N
UseSynthetic BiologyTagsmKateExpressionMutationPromoterT7-lac inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only