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Plasmid#108795PurposeExpresses N-terminus to aa571 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa571
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA
Plasmid#108796PurposeExpresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa572 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRELA
Plasmid#83944PurposeLentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.DepositorInsertsgRELA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmNanoLuc
Plasmid#182969PurposeStably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmNanoLuc
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Full human ELL2
Plasmid#127253PurposeExpresses full-length human ELL2 in mammalian cellsDepositorInsertELL2 (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationPromoterpEF1aAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH1-MGT#2-mCherry
Plasmid#164097PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#2-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSRW1JZ
Plasmid#129528PurposeC. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pairDepositorInsertFlincG3
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABEMax nSaCas9
Plasmid#135364PurposeExpresion of SaCas9 nickase (D10A) with ABEMax at the N terminusDepositorInsertABEMax nSaCas9
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#108769PurposeExpresses N-terminus to aa396 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330 N-FKBP-Cas9
Plasmid#162727PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-H159Q-HA
Plasmid#198183PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) H159QDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe histidine in IRG1 at position 159 mutates to …PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLpA-DN.JNK2
Plasmid#51943PurposeAdenoviral vector for dominant negative JNK2 expressionDepositorInsertDN JNK2 (Mapk9 Mouse)
UseAdenoviralTagsHAExpressionMutationdominant negative: the dual phosphorylation moti…PromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FH1-PNGT#1-mCherry
Plasmid#164098PurposeFluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.DepositorInsertPNGT#1-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mNeonGreen-CI
Plasmid#196850PurposeExpression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoterDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA
Plasmid#108814PurposeExpresses aa327 to C-terminus of TP901 integrase fused to FKBPDepositorInsertTP901 integrase fragment aa327 to C-terminus
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 NC-FKBP-Cas9
Plasmid#162725PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64T acvr1ba*
Plasmid#185409PurposeConstitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)DepositorInsertacvr1ba (acvr1ba Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Threonine 206 to Aspartic acidPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP
Plasmid#196875PurposeNeuron-specific expression of LynGFP reporterDepositorInsertLynEGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only