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Plasmid#184886PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pECNUS1
Plasmid#184884PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO
Plasmid#192382PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.DepositorInsertAct2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3
Plasmid#192397PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).DepositorInsert35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130PromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10
Plasmid#218866PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry
Plasmid#35510PurposeAAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterEf1aAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218863PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-GFP(39TAG)
Plasmid#217360PurposeExpression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmidDepositorInsertEnhanced green fluorescent protein
UseBaculoviralTagsExpressionInsect and MammalianMutationY39TAGPromoterCMVAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
Plasmid#35512PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218864PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.PHF19
Plasmid#125168PurposeExpresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPHF19 (PHF19 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
Plasmid#35511PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10
Plasmid#218867PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123A)-EYFP
Plasmid#35507PurposeAAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123APromoterEf1aAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123A)-mCherry
Plasmid#35506PurposeAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorHas ServiceAAV9InserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123APromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123A)-EYFP
Plasmid#35505PurposeAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorHas ServiceAAV9InserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123APromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218865PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only