We narrowed to 14,439 results for: NTS;

1. XE5 Xwnt-5A Hind RNA

   Plasmid

   #16872

   Depositor

   Randall Moon

   Article

   Moon et al Development. 1993 Sep. 119(1):97-111.

   Insert

   Xwnt-5A Hind RNA (wnt5a.S Frog)
   

   Use

   Xenopus expression

   Mutation

   Starts with AA 14 (see comments)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pH-ePPE

   Plasmid

   #183097

   Purpose

   For plant prime editing in rice plants

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. iLID-GFP-GCN4-ppKin14

   Plasmid

   #240420

   Purpose

   -end oriented kinesin for optogenetic pulling experiments

   Depositor

   Anna Akhmanova

   Article

   Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

   Insert

   ppKin14Vib
   

   Tags

   GCN4, GFP, and iLID

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-850

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. P307-FBP_6

   Plasmid

   #139697

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEmpty_Control

   Plasmid

   #162607

   Purpose

   Empty control for Cas9 experiments and to clone new guide RNAs for paper

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. P362-FBP_12

   Plasmid

   #139703

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ePPE-spG

   Plasmid

   #183096

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nspGCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpGCas9

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. P427-FBP_13

   Plasmid

   #139704

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

   Plasmid

   #136627

   Purpose

   Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-R59
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19/Tp-mChe

    Plasmid

    #183165

    Purpose

    Construct for transient expression of tonoplast-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Tp-mCherry
    

    Tags

    28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ePPE

    Plasmid

    #183095

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGreen_dualluc_3'UTR_sensor

    Plasmid

    #55206

    Purpose

    Dual-luciferase reporter for microRNA targeting in plants

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Luciferase; Plant transient expression

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19/Pt-mChe

    Plasmid

    #183164

    Purpose

    Construct for transient expression of plastids-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Pt-mCherry
    

    Tags

    57 aa of the Pisum sativum rubisco small subunit …

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. puc19-pCas13a

    Plasmid

    #118963

    Purpose

    Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

    Depositor

    Magdy Mahfouz

    Article

    Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

    Insert

    Cas13a
    

    Expression

    Plant

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ151-HDR

    Plasmid

    #113631

    Purpose

    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

    Depositor

    Alex Kentsis

    Inserts

    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    

    Use

    Cre/Lox; Homology recombination selection vector

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a promoter, EF1a promoter (T2A cleaving sequen…

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19/mChe-Px

    Plasmid

    #183170

    Purpose

    Construct for transient expression of peroxisomes-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Px
    

    Tags

    10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUbi-MAD7

    Plasmid

    #170133

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    maize Ubiquitin-1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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