We narrowed to 4,232 results for: pCMV

1. pCMV-dCas12a-A3A-Y132D

   Plasmid

   #193641

   Purpose

   Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-Y132D
   

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-Y132D

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV/myc/Nuc-hOGG1

   Plasmid

   #18709

   Depositor

   David Sidransky

   Article

   Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

   Insert

   8-oxoguanine DNA glycosylase (OGG1 Human)
   

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-RVR-A3A-Y130F

   Plasmid

   #193658

   Purpose

   Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-Y130F, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-RVR-A3A-N57G

   Plasmid

   #193657

   Purpose

   Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-N57G, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Sport6-CD81-pHuji

   Plasmid

   #130904

   Purpose

   Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD81-pHuji (CD81 Synthetic, Human)
   

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Leu-49 and Gly-50 in extra…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-FRB-tTA-Myc

   Plasmid

   #177911

   Purpose

   FRB-tTA-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   FRB-tTA
   

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hA3A-eBE-Y130F

   Plasmid

   #113423

   Purpose

   Expresses hA3A-eBE-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3XFlag pCMV5-TOPO TAZ WT

   Plasmid

   #24809

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCMV HA hRb delta CDK

    Plasmid

    #58906

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-MMLV-gag-hMLH1dn

    Plasmid

    #195513

    Purpose

    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn (MLH1 Human)
    

    Use

    Virus like particle

    Tags

    FLAG, NES, and Protease cleavate site

    Expression

    Mammalian

    Mutation

    Human MLH1 del754-756

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SPORT6-RNF114-RINGmut

    Plasmid

    #69922

    Purpose

    Expresses mutant myc-RNF114 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Expression

    Mammalian

    Mutation

    C29A, C32A

    Promoter

    CMV

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid

    #224123

    Purpose

    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Q20A_Y23A_Y24F_F35A in La(SSB)1-194

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-XL4 FLAG-SFRP2-Fc

    Plasmid

    #115772

    Purpose

    Expression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    SFRP2 (SFRP2 Human)
    

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-Flag-EPLIN beta

    Plasmid

    #40929

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN beta (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Neo-Bam E2F3a

    Plasmid

    #37970

    Depositor

    Jacqueline Lees

    Article

    Lees et al Mol Cell Biol. 1993 Dec;13(12):7813-25.

    Insert

    E2F3a (E2F3 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-AID-BLM-UGI

    Plasmid

    #221218

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI
    

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV/myc/mito-hOGG1

    Plasmid

    #18706

    Depositor

    David Sidransky

    Article

    Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

    Insert

    8-oxoguanine DNA glycosylase (OGG1 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-ABE7.10-P2A-GFP

    Plasmid

    #162509

    Purpose

    Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

    Insert

    ABE7.10-P2A-GFP
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-FLAG-LOV*-H2B

    Plasmid

    #202204

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Histone H2B type 1-K
    

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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