We narrowed to 10,632 results for: t7

1. pOpen-T7gene5

   Plasmid

   #165534

   Purpose

   DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   T7 DNA Polymerase (unmodified)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MZB174

   Plasmid

   #228487

   Purpose

   10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Type

   Empty backbone

   Tags

   10xHis-SUMO(Smt3)-GST-HRV

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET PPL His6 LIC cloning vector (2J-T)

   Plasmid

   #37182

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6 and PPL

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK210

   Plasmid

   #73325

   Purpose

   Produces Janthinobacterium lividum violacein biosynthesis protein VioD

   Depositor

   T. J. Kappock

   Insert

   violacein biosynthesis protein VioD
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. BRPF1B-c001

   Plasmid

   #53620

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   BRPF1B
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. ETV4A-c072

   Plasmid

   #53638

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   ETV4A
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET15-EF1p_mFAP2b

   Plasmid

   #159469

   Purpose

   Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1p_mFAP2b
   

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK515

   Plasmid

   #72385

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylglycinamide formyltransferase 2
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BRD2A-c014

   Plasmid

   #53626

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD2A
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pETM41-EcPPK

    Plasmid

    #38334

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, MBP, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pET15-EF4n_mFAP2b

    Plasmid

    #159464

    Purpose

    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF4n_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15-EF1p3_mFAP2b

    Plasmid

    #159470

    Purpose

    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1p3_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15-mFAP10

    Plasmid

    #159463

    Purpose

    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    mFAP10
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM263-EcPPK

    Plasmid

    #38333

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK100

    Plasmid

    #73264

    Purpose

    Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylformylglycinamidine synthase subunit PurS
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC23-LIC-STREP

    Plasmid

    #182507

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal STREP

    Depositor

    Renato Bruni

    Type

    Empty backbone

    Tags

    TEV-STREP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SnFR_S074

    Plasmid

    #182811

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    S074-CholineSnFR
    

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC28-LIC-His

    Plasmid

    #182504

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal His

    Depositor

    Renato Bruni

    Type

    Empty backbone

    Tags

    TEV-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET LIC cloning vector for hairpin mRNA (2U-T)

    Plasmid

    #29719

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    MYFQSNA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. SnFR_S062

    Plasmid

    #182810

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    S062-CholineSnFR
    

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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