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Plasmid#227757PurposeExpresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationGly80Glu mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG123
Plasmid#227755PurposeExpresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationAla157Val mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG143
Plasmid#227754PurposeExpresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationLeu87IlePromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG147
Plasmid#227753PurposeExpresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationGly94Cys mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG120
Plasmid#227752PurposeExpresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationAsp89Tyr mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG137
Plasmid#227751PurposeExpresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationVal201Leu mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG136
Plasmid#227750PurposeExpresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationPro165Ser mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG135
Plasmid#227749PurposeExpresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationLys99Glu mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG119
Plasmid#227748PurposeExpresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationVal139Leu mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG118
Plasmid#227747PurposeExpresses a version of light-inducible transcription factor EL222 (Asp18Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationAsp18Tyr mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG117
Plasmid#227746PurposeExpresses a version of light-inducible transcription factor EL222 (Asp18Tyr, Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationAsp18Tyr, Val139Leu double mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJS237
Plasmid#200713PurposeADH2 terminator from yeast (MoClo 3´U +Ter)DepositorInsertADH2 Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 6FLAG
Plasmid#182509PurposeC-SWAT compatible vector for C-terminal tagging with 6 FLAGDepositorInsert6 FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP
Plasmid#182510PurposeC-SWAT compatible vector for C-terminal tagging with GFPDepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_E2Aq(2nd)
Plasmid#190911Purpose2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertE2A
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2Aq(2nd)
Plasmid#190918Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2Aq(1st)
Plasmid#190912Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2Aq(1st)
Plasmid#190917Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2Ab
Plasmid#183084Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2Aq(2nd)
Plasmid#183089Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIR3DH8K
Plasmid#165069PurposeKnocking out GAL80 using KanMX4 markerDepositorInsertgal80Arm1-P-TPI1-KanMX4-gal80Arm2
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ECM_unstable
Plasmid#127607Purposehigh-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3DepositorInsertUnstable ECM33 intron (Mut)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ECM33_intron
Plasmid#127596Purposelow-copy URA-selectable plasmid, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-RPL27B_intron
Plasmid#127604Purposelow-copy URA-selectable plasmid, RPL27B intron inserted into URA3DepositorInsertRPL27B intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-APS3_intron
Plasmid#127605Purposelow-copy URA-selectable plasmid, APS3 intron inserted into URA3DepositorInsertAPS3 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HNT2_intron
Plasmid#127603Purposelow-copy URA-selectable plasmid, HNT2 intron inserted into URA3DepositorInsertHNT2 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV16
Plasmid#128894PurposePosition 6 module- LEU2DepositorInsertLEU2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV22
Plasmid#128884PurposePosition 3 module- VenusDepositorInsertVenus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV42
Plasmid#128888PurposePosition 4 module- mCherry-STOPDepositorInsertmCherry+STOP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV44
Plasmid#128889PurposePosition 4 module- sfGFP-STOPDepositorInsertsfGFP+STOP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only