We narrowed to 7,286 results for: lenti crispr

1. pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170816

   Purpose

   Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mScarlet-I-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170815

   Purpose

   Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mScarlet-I-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

   Plasmid

   #134252

   Purpose

   Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 179-219, and mutated coding s…

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-Cre stuffer v4

   Plasmid

   #158033

   Purpose

   Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BRD4 CRISPRi plasmid

   Plasmid

   #154890

   Purpose

   Human BRD4 CRISPRi gRNA

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   BRD4-targeting gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

   Plasmid

   #216171

   Purpose

   Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170810

   Purpose

   Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170812

    Purpose

    Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-CMV-MLH1dn-IRES-puroR

    Plasmid

    #207354

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    MutL homolog 1 (MLH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Δ754-756 dominant negative mutant

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid

    #207355

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Non-targeting CRISPRi plasmid

    Plasmid

    #154891

    Purpose

    Human non-targeting CRISPRi gRNA

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

    Insert

    Nontargeting gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

    Plasmid

    #177120

    Purpose

    dCasRx mediated RNA tethering of FTO

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    dCasRx-FTO
    

    Use

    Lentiviral

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    dCasRX contains R239A/H244A/R858A/H863A mutations…

    Promoter

    Ef1a

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO5-SgHottip-GFP-CRISPRa

    Plasmid

    #134990

    Purpose

    dCas9-mediated activation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO5-SgHottip-GFP-CRISPRi

    Plasmid

    #134989

    Purpose

    dCas9-mediated inactivation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOT_2 - lenti-EFS-tNGFR-2A-puro

    Plasmid

    #181971

    Purpose

    Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    tNGFR (NGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    truncation: aa 11-276

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

    Plasmid

    #216166

    Purpose

    Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    phU6, ph7SK, phH1, pmU6

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

    Plasmid

    #238169

    Purpose

    Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    CD45 sgRNA CRISPRa (PTPRC Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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