We narrowed to 155,984 results for: ins
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pFX-Venus-Sec61β
Plasmid#174467PurposeExpress Venus with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
TagsSec61 subunit betaExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-iRFP
Plasmid#175830PurposeExpress iRFP with microtubule-targeting sequence in mammalian cellsDepositorInsertiRFP with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-SECFP
Plasmid#175826PurposeExpress SECFP with microtubule-targeting sequence in mammalian cellsDepositorInsertSECFP with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectPromoterPolyhedrinAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87E
Plasmid#36057DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
TagsPEST and VAMP 3 UTRExpressionMammalianPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-KRasCT
Plasmid#175833PurposeExpress EGFP with plasma membrane-targeting sequence in mammalian cellsDepositorInsertEGFP with plasma membrane-targeting sequence
TagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
CBP-Bromo-HAT-CH3-sp65
Plasmid#21026DepositorInsertCBP (Crebbp Mouse)
UseIn vitro tntTagsFlagMutationBromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…Available SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-Sec61β
Plasmid#174468PurposeExpress mCherry with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
TagsSec61 subunit betaExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVenus_Lys-less-Cdc20 (1620)
Plasmid#39849DepositorInsertCdc20 (CDC20 Human)
TagsVenusExpressionMammalianMutationLys-less = all Lys converted to ArgAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-Sirius
Plasmid#175825PurposeExpress Sirius with microtubule-targeting sequence in mammalian cellsDepositorInsertSirius with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-SECFP
Plasmid#174470PurposeExpress SECFP with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertSECFP with golgi apparatus-targeting sequence
Tagsβ1,4-galactose transferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDB181
Plasmid#80692Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06DepositorInsertgEHEE_06
TagsOsmY-10His-Smt3ExpressionBacterialPromoterT7Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-iRFP
Plasmid#174474PurposeExpress iRFP with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertiRFP with golgi apparatus-targeting sequence
Tagsβ1,4-galactose transferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-iRFP
Plasmid#175823PurposeExpress iRFP with actin-targeting sequence in mammalian cellsDepositorInsertiRFP with actin-targeting sequence
TagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6595 MSCV-IP-N-HA-GPRC5B
Plasmid#34894DepositorAvailable SinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LacI/109-pHG165c
Plasmid#90058PurposeWT LacI polymorphism with T at position 109DepositorInsertLacI/109
ExpressionBacterialMutation109PromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBC2-PuroIII
Plasmid#153420PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralExpressionMammalianAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RCK-Y1699C
Plasmid#25067DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains only the ROC-COR-Kinase domains aa 1329…Available SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
M72-IRES-tauRFP2-ACNF TV
Plasmid#15512DepositorInsertM72-IRES-tauRFP2-ACNF TV (Olfr160 Mouse)
UseMouse TargetingMutationTargeting vector to insert IRES-tauRFP2-ACNF imme…Available SinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
TagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) HIS-tag pET
Plasmid#8492DepositorInsertHoxA9 (Hoxa9 Mouse)
TagsHis and T7ExpressionBacterialMutationBamHI/BalI fusion into amino acid #2 of HOXA9; ma…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-Venus
Plasmid#174478PurposeExpress Venus with actin-targeting sequence in mammalian cellsDepositorInsertVenus with actin-targeting sequence
TagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-Venus
Plasmid#174472PurposeExpress Venus with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertVenus with golgi apparatus-targeting sequence
Tagsβ1,4-galactose transferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-KRasCT
Plasmid#175834PurposeExpress Venus with plasma membrane-targeting sequence in mammalian cellsDepositorInsertVenus with plasma membrane-targeting sequence
TagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) HIS-tag pET
Plasmid#8553DepositorInsertHOXD12 (Hoxd12 Mouse)
TagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATGAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only
