We narrowed to 4,300 results for: Pet2

1. TTR C10S/S85C/T119M

   Plasmid

   #242712

   Purpose

   bacterial expression of TTR

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, T119M

   Promoter

   T7

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TTR C10S/S85C/V122I

   Plasmid

   #238122

   Purpose

   bacterial expression of TTR

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, V122I

   Promoter

   T7

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TTR C10S/S85C/F87A

   Plasmid

   #238120

   Purpose

   bacterial expression of TTR

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, F87A

   Promoter

   T7

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TTR C10S/S85C/V30M

   Plasmid

   #238119

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, V30M

   Promoter

   T7

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TTR C10S/S85C/L55P

   Plasmid

   #238121

   Purpose

   bacterial expression of TTR

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S85C, L55P

   Promoter

   T7

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TTR A120S

   Plasmid

   #238111

   Purpose

   bacterial expression of human TTR mutant

   Depositor

   Peter Wright

   Article

   Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A120S

   Promoter

   T7

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TTR A120L

   Plasmid

   #238110

   Purpose

   bacterial expression of human TTR mutant

   Depositor

   Peter Wright

   Article

   Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A120L

   Promoter

   T7

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TTR A25T

   Plasmid

   #238114

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A25T

   Promoter

   T7

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His6-tev-G(TTR A25T)

   Plasmid

   #238116

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   His6tev-G

   Expression

   Bacterial

   Mutation

   A25T

   Promoter

   T7

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLuc-C-HiBiT

    Plasmid

    #207118

    Purpose

    Bacterially expressed FLuc-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    luciferin 4-monooxygenase
    

    Use

    Tags

    HiBiT Tag and His-tag with thrombin site

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLuc-C-NLuc

    Plasmid

    #234566

    Purpose

    Bacterially expressed FLuc-C-NLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Firefly luciferase
    

    Use

    Tags

    NanoLuciferase-thrombin site-10xHis and T7 gene 1…

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MZB220

    Plasmid

    #221771

    Purpose

    Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    Arl1 (ARL1 Human)
    

    Use

    Tags

    CPD

    Expression

    Bacterial

    Mutation

    Q71L

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MZB222

    Plasmid

    #221772

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    ArfGEF1 (ARFGEF1 Human)
    

    Use

    Tags

    10xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TTR C10S S85C G83R

    Plasmid

    #184747

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, G83R

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TTR C10S S85C Y78F

    Plasmid

    #184746

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, Y78F

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TTR C10S S85C A81T

    Plasmid

    #184745

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, A81T

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TTR C10S S85C K80E

    Plasmid

    #184744

    Purpose

    bacterial expression of huamn TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80E

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SNAP-Kindlin

    Plasmid

    #186136

    Purpose

    For expression of recombinant Snap-labeled kindlin in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    FERMT2 (FERMT2 Human)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TTR C10S S85C I84S

    Plasmid

    #184748

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, I84S

    Promoter

    T7

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK516

    Plasmid

    #71578

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to glutamine

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJK524

    Plasmid

    #71580

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes asparagine-347 to alanine

    Promoter

    T7

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK513

    Plasmid

    #71576

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-294 to alanine

    Promoter

    T7

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK517

    Plasmid

    #71579

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to aspartate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK514

    Plasmid

    #71577

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamine-435 to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-GST-TNRC6A-(935-984)-NES-mut

    Plasmid

    #42051

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    contain aa935-984, F951A, F955A, I958A, F960A

    Promoter

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-MRPL15

    Plasmid

    #31345

    Depositor

    Linda Spremulli

    Article

    Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

    Insert

    mitochondrial ribosomal protein L15 mature form (MRPL15 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Has the amino acids in the predicted mature form …

    Promoter

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AB-GFP

    Plasmid

    #72203

    Purpose

    screen AB42 variants for aggregation

    Depositor

    Michael Hecht

    Article

    Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

    Insert

    AB42 (APP Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. OPEN HLA-A*02:01-BirA

    Plasmid

    #215569

    Purpose

    E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    Glycine 120 to Cysteine

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

    Plasmid

    #89508

    Purpose

    Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

    Insert

    rAPOBEC1-nCas9 (D10A)-UGI-NLS
    

    Use

    Tags

    UGI and rAPOBEC-XTEN

    Expression

    Bacterial

    Mutation

    D10A mutation of Cas9

    Promoter

    T7

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEE075

    Plasmid

    #176832

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    714
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TTR C10S S46C

    Plasmid

    #190008

    Purpose

    Expression of human TTR in E coli

    Depositor

    Peter Wright

    Article

    Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S46C

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEE050

    Plasmid

    #176807

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    601
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEE060

    Plasmid

    #176817

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    611
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEE065

    Plasmid

    #176822

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    704
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEE062

    Plasmid

    #176819

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    701
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEE077

    Plasmid

    #176834

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    716
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. ALARM NMR

    Plasmid

    #90209

    Purpose

    Expresses the human La antigen for use in ALARM NMR

    Depositor

    Michael Walters

    Article

    Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

    Insert

    La antigen (SSB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    contains amino acids 100-324

    Promoter

    T7 promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRD421

    Plasmid

    #163112

    Purpose

    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    

    Use

    Tags

    His-tag with TEV-cleavable linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only