We narrowed to 10,184 results for: tre promoter

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U6-hGRIN2B-CAG-ps-SpCas9

Plasmid

#102851

Purpose

A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. pyogenes)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-C2m2-SNAP

Plasmid

#208791

Purpose

For AAV production to express C2m2-SNAP in astrocytes

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Use

AAV

Tags

SNAP-tag

Expression

Mammalian

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

GFAP promoter

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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lentiMutB3GNT5-blast

Plasmid

#208380

Purpose

lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

nt 922 c->g (PAM site inactivation)

Promoter

EF-1 alpha core promoter

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

Plasmid

#117856

Purpose

This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, S. aureus, Synthetic)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Promoter

U6 promoter;

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-Rosmarinus

Plasmid

#183462

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

Rosmarinus

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mCarmine

Plasmid

#183464

Purpose

To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mCarmine

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-CTLA4-FLN-ELP-His-ybbr

Plasmid

#168038

Purpose

Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)

Tags

3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pIS1 ATP2B1

Plasmid

#60788

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

ATP2B1 3'UTR and wild-type miR-155 binding site (ATP2B1 Human)

Use

Luciferase

Expression

Mammalian

Promoter

thymidine kinase (HSV-TK promoter)

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Ttr

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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