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pBac(3xP3-gTc’v; Tc-hsp68:Cre)

Plasmid

#119063

Purpose

Expression of Cre recombinase in Tribolium castaneum

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
Tc-hsp68 Promoter
Cre recombinase
Tc-hsp68 3'UTR
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Expression

Insect

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1003

Plasmid

#71821

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML2-MYC

Plasmid

#18829

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

Plasmid

#20293

Purpose

Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Alanine

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046494

Plasmid

#149137

Purpose

Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDS250

Plasmid

#34969

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V416I

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCR1055

Plasmid

#71823

Purpose

Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS

Use

CRISPR

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pExodus

Plasmid

#39991

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CMV, T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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