Purpose
Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
Insert
EF1a-Puro-WPRE-hU6-gRNA
Use
CRISPR and Lentiviral
Available Since
Feb. 9, 2017
Availability
Academic Institutions and Nonprofits only