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CMV-pDisplay-SNAP[C145A]

Plasmid

#223496

Purpose

Expression of dead-mutant SNAP-tags to outer surface in mammalian cells

Depositor

James Frank

Article

Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

Insert

dead-mutant SNAP-tag

Expression

Mammalian

Mutation

SNAP-tag C145A

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV:: GFP11x7-P2A-NES-FKBP-iLID-LRP6c(△1-64)

Plasmid

#221777

Purpose

Expresses GFP11x7-P2A-NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: GFP11x7-P2A-NES-FKBP-iLID-LRP6c(△1-64)

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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p4A8_M59I_T94M_BC

Plasmid

#217833

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Expression

Yeast

Mutation

VH: M59I, VL: T94M

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBDP

Plasmid

#217827

Purpose

Stuffer plasmid with golden gate compatible bidirectional promoter

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

Gal1/Gal10 Bidirectional Promoter

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-FRB-Rab5

Plasmid

#214272

Purpose

Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.2

Plasmid

#191878

Purpose

Encodes a GFP design, bleach.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.2

Tags

His Tag

Expression

Bacterial

Mutation

V61L T65S V68A S72A V112I Y145M

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mid.8

Plasmid

#191902

Purpose

Encodes a GFP design, mid.8

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.8

Tags

His Tag

Expression

Bacterial

Mutation

V61A S72T Y145M L220V

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1954

Plasmid

#195689

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A/L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1936

Plasmid

#195692

Purpose

Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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