We narrowed to 17,082 results for: EMB

1. GGE252

   Plasmid

   #120791

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE253

   Plasmid

   #120790

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pyb1301(JBEI-11629)

   Plasmid

   #87959

   Purpose

   jStack yeast assembly into plant vectors, Level 2 pYB Vector, Hygro selection

   Depositor

   Dominique Loque

   Article

   Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG-D-SpyTag-His6-E

   Plasmid

   #87375

   Purpose

   An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   SpyTag
   

   Use

   Synthetic Biology

   Tags

   His6 tag

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Guide-Generating Vector

   Plasmid

   #125752

   Purpose

   Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

   Insert

   Cys4-GFPdropout-SpCas9scaffold
   

   Use

   Synthetic Biology

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EB3-eGFP-2xC2-iLID

   Plasmid

   #114419

   Purpose

   anchor iLID domain to plasma membrane with slower diffusion

   Depositor

   Dorus Gadella

   Article

   Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

   Insert

   iLID domain
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GGE209

   Plasmid

   #120793

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp-Mfe
   

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7T-CcadTSMod

   Plasmid

   #99874

   Purpose

   RNA synthesis for microinjection into xenopus laevis embryo

   Depositor

   Marc Tramier

   Article

   Herbomel et al Sci Rep. 2017 Mar 22;7:45058. doi: 10.10

   Insert

   CcadTSMod
   

   Use

   Xenopus microinjection

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCG-B-mSpyCatcher-C

   Plasmid

   #87374

   Purpose

   An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   mSpyCatcher
   

   Use

   Synthetic Biology

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCG-A-celA(SP)-B

    Plasmid

    #87371

    Purpose

    An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    CelA signal peptide
    

    Use

    Synthetic Biology

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCG-A-xynA(SP)-B

    Plasmid

    #87372

    Purpose

    An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    XynA signal peptide
    

    Use

    Synthetic Biology

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQuantA-hygro-amp

    Plasmid

    #45584

    Depositor

    Andrzej Dziembowski

    Article

    Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

    Type

    Empty backbone

    Use

    Cre/Lox; Dt40 cell line targeting

    Tags

    Quant-A tag

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTH646-2µ-RLuc

    Plasmid

    #40600

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    Renilla Luciferase
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046268

    Plasmid

    #145423

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4046216

    Plasmid

    #145490

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. GGE094

    Plasmid

    #120787

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GGE091

    Plasmid

    #120786

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GGE254

    Plasmid

    #120789

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GGE255

    Plasmid

    #120788

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR Bmi1-C

    Plasmid

    #30511

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Gateway - donor vector

    Mutation

    NO STOP

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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