We narrowed to 14,168 results for: eng
-
Plasmid#171152PurposepGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassetteDepositorInsertJ3-BBa_J23117-mRFP
ExpressionBacterialPromoterJ3-BBa_J23117Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC034
Plasmid#171153PurposepGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassetteDepositorInsertJ3(106)- BBa_J23111-sfGFP
ExpressionBacterialPromoterJ3(106)-BBa_J23111Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC018.AAV
Plasmid#171146PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC016.109
Plasmid#171144PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC030.306
Plasmid#171151PurposeMevalonate production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mvaES
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
ExpressionYeastPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMY07AB-PRO
Plasmid#127710PurposeLevel 0 destination vector for a promoter with A - B scars. Clone in with BpiIDepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala13
Plasmid#87174PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
ExpressionBacterialMutationD196A, L197RPromoterConstitutive proDAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala11
Plasmid#87172PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
ExpressionBacterialMutationD196A, L197RPromoterConstitutive proAAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala12
Plasmid#87173PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
ExpressionBacterialMutationD196A, L197RPromoterConstitutive proCAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTCW015
Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
ExpressionYeastPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
PromoternoneAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-3
Plasmid#233295PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
Promoterp14GAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-3
Plasmid#233296PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7 and unknownAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_TRE-mScarlet
Plasmid#229796PurposeBxb1-GT donor plasmid for doxycycline-inducible mScarlet expressionDepositorInsertmScarlet
UseSynthetic BiologyExpressionMammalianPromoterTREAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHC027 (∆pemK)
Plasmid#226204PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.DepositorInsertUpstream/downstream homology arms for addiction system toxinPemK.
UseSynthetic BiologyAvailable sinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY128
Plasmid#127713PurposeLevel 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
Plasmid#126739Purposeyeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2, acs1*, ald6
Tags6xHis,MycExpressionYeastMutationT3542C and A30S, S629N (please see depositor comm…PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ACS1
Plasmid#126712Purposeyeast plasmid complementing ACS1 from Komagataella phaffiiDepositorInsertacs1
Tags6xHis,MycExpressionYeastPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only