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pPOTv6-blast-His:mScarletI:His

Plasmid

#201097

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 HCoV-OC43 NSP5_nostop

Plasmid

#168690

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP5 (ORF1ab )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-OC43 NSP12_nostop

Plasmid

#168696

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP12 (ORF1ab )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-NL63 E_stop

Plasmid

#168684

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-NL63 E_nostop

Plasmid

#168666

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 MERS-CoV NSP6_stop

Plasmid

#168633

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (orf1ab )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 MERS-CoV NSP15_stop

Plasmid

#168640

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP15 (orf1ab )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarletI

Plasmid

#179822

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarlet

Plasmid

#179823

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 MERS-CoV NSP3_nostop

Plasmid

#168608

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP3 (orf1ab )

Use

Other

Available since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-HKU1 NSP1_nostop

Plasmid

#168570

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP1 (ORF1ab )

Use

Other

Available since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-1 NSP6_stop

Plasmid

#168548

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (ORF1ab )

Use

Other

Available since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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E2_pENTR_R4R3_T CTP

Plasmid

#171736

Purpose

ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

Depositor

Shin-ichi Arimura

Publication

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal

Use

Multisite gateway entry vector

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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DT036A

Plasmid

#159396

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Publication

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tet promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-17

Plasmid

#160717

Purpose

TrpC Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Publication

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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