164,609 results

1. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   P45
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. GLP1R-DuET

   Plasmid

   #213253

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GLP1R (GLP1R Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Promoter

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3` CC: Hygro – GFP-N – loxP – mScarlet

   Plasmid

   #219564

   Purpose

   3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   3` CC: Hygro – GFP-N – loxP – mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF1-a

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-TO-dCas9-3XmCherry

   Plasmid

   #64108

   Purpose

   Expression of Sp dCas9-3XmCherry in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCM157

   Plasmid

   #45863

   Depositor

   Mary Lidstrom

   Article

   Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

   Insert

   cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mammalian expression: Z-lock cofilin dark state mutant

   Plasmid

   #141136

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP VARP

   Plasmid

   #42312

   Depositor

   Thierry Galli

   Article

   Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

   Insert

   VARP (ANKRD27 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. C-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58694

   Purpose

   Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Csplit Intein and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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