We narrowed to 891 results for: TDT

1. pBa-flag-BicD2 594-FKBP

   Plasmid

   #64206

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Use

   Tags

   FKBP and flag

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDragon-Ctgf

   Plasmid

   #155015

   Purpose

   For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

   Depositor

   Alberto Rosello-Diez

   Article

   Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

   Insert

   Ccn2 (Ccn2 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   M24T (please see depositor comments)

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-tdPCP-CIBN

   Plasmid

   #183937

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-CIBN (CIB1 Synthetic, Mustard Weed)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2705-AGER-eGFP

   Plasmid

   #216474

   Purpose

   donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC_dCas9_sgRNA Td1

   Plasmid

   #176257

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

   Plasmid

   #198753

   Purpose

   Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. eMscL G22S

   Plasmid

   #107455

   Purpose

   eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Mutation

   Bearing a glycine to serine substitution at posit…

   Promoter

   human synapsin 1

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

   Plasmid

   #133785

   Purpose

   Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

   Depositor

   Michael Stryker

   Article

   Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

   Insert

   PSD95-EGFP (Dlg4 Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. INS-GFP-CD19-EPT

    Plasmid

    #210469

    Purpose

    Donor plasmid for knock-in GFP-CD19 into the human INS locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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