We narrowed to 115,050 results for: CIL

1. FUW TetO NET1A-V5 S46A

   Plasmid

   #69829

   Purpose

   Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
   

   Use

   Lentiviral

   Tags

   v5

   Expression

   Mammalian

   Mutation

   S46A

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONOR-SNCAe3-WT

   Plasmid

   #85847

   Purpose

   Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   SNCA exon 3 homology arms (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-FLAG-CHD5-WT-331-672aa

   Plasmid

   #68870

   Purpose

   Mammalian expression of CHD5 histone binding domain (331-672aa)

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   331-672aa

   Promoter

   CMV

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA TMEM 138-flag

   Plasmid

   #41628

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA5-TEX264-SIM1_mut-cGFP

   Plasmid

   #220212

   Purpose

   Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

   Plasmid

   #207344

   Purpose

   Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Δ37, Δ115-133, Δ204-217

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBpuro-EGFP-eDHFR(69K6)-cRaf

   Plasmid

   #178849

   Purpose

   Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-cRaf (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBpuro-cRaf-mNG-eDHFR(69K6)

   Plasmid

   #172107

   Purpose

   Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. QuasAr-P2A-hKvb1

   Plasmid

   #154083

   Purpose

   Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

   Depositor

   Michael Hoppa

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

   Insert

   QuasAr-P2A-human Kvb1 (KCNAB1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   myc-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONOR-SNCAe2-WT

    Plasmid

    #85845

    Purpose

    Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Insert

    SNCA exon 2 homology arms (SNCA Human)
    

    Use

    Tags

    -

    Expression

    Bacterial and Mammalian

    Mutation

    -

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN459

    Plasmid

    #137875

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONOR-SNCAe3-A53T

    Plasmid

    #85848

    Purpose

    Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Insert

    SNCA exon 3 homology arms (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-GST-CHD5-L518A/Y619E-FRAG

    Plasmid

    #68874

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    331-672aa, L518A/Y619E

    Promoter

    tac

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET-EIN-FTO(32-505)

    Plasmid

    #186466

    Purpose

    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    Fat mass obesity associated protein (FTO Human)
    

    Use

    Tags

    6 Histidine-EIN (N-terminal domain of E.coli Enzy…

    Expression

    Bacterial

    Mutation

    amino acid 32-505

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. gCBEv3

    Plasmid

    #220618

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GABA(A) receptor subunit a2SE

    Plasmid

    #49169

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    

    Use

    Tags

    HA, bovine alpha 1 signal sequence, and pHGFP (pH…

    Expression

    Mammalian

    Mutation

    T343A

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid

    #129415

    Purpose

    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    RAB18 HDR template (RAB18 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. gTBEv4

    Plasmid

    #220620

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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