We narrowed to 5,913 results for: pcDNA 3.1

1. pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

   Plasmid

   #177768

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S95A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

   Plasmid

   #177769

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K116A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

   Plasmid

   #177770

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T139A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

   Plasmid

   #177771

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K370A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

   Plasmid

   #177772

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S374A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

   Plasmid

   #177773

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K592A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MS2-HA-HsSmg5-G120E_W

   Plasmid

   #147819

   Purpose

   Mammalian Expression of HsSmg5-G120E

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-G120E (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

   Plasmid

   #147558

   Purpose

   Mammalian Expression of HsSmg7_633-1091

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg7_633-1091 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation V854I compared to the seq…

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-myc-TRBP-dsRBD2

   Plasmid

   #180602

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-myc-TRBP-dsRBD1

    Plasmid

    #180601

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid

    #180760

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Asp115Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Asp115Ala, Leu…

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-TRBP-L2+D2+L3-myc/His

    Plasmid

    #180600

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Flag-gp130dimer-stm2585

    Plasmid

    #174395

    Purpose

    Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain and sarA chimera
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Flag-stm2585-gp130

    Plasmid

    #174393

    Purpose

    Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    140-177 of sarA replaced with homologous IL6ST se…

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-mas-PRGRH-Nluc

    Plasmid

    #158608

    Purpose

    Plasmid expressing PRG(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-mas-p115RH-Nluc

    Plasmid

    #158607

    Purpose

    Plasmid expressing p115(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 mitoAURKA Lys162Met

    Plasmid

    #157752

    Purpose

    Expression of kinase-dead AURKA K62M in mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1 AURKA deltaNter

    Plasmid

    #157749

    Purpose

    Expression of AURKA without Nter in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    the first 30AA of Aurora A are lacking

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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