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pFM109

Plasmid

#40155

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, C56S (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM108

Plasmid

#40154

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, S241A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM106

Plasmid

#40152

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R163A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM105

Plasmid

#40151

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, K162A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM104

Plasmid

#40150

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R183-5A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded …

Promoter

T7

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEG495

Plasmid

#40089

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S404A, S458A) (mex-5 Nematode)

Tags

MBP

Expression

Bacterial

Mutation

S404A, S458A

Promoter

P-lac

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pEG268

Plasmid

#40075

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S458A) (mex-5 Nematode)

Tags

MBP

Expression

Bacterial

Mutation

S458A

Promoter

P-lac

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pFM116

Plasmid

#40162

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C+S950A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa840-1268); S950A

Promoter

tac

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pFM052

Plasmid

#40146

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (C56S) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

pie-1

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pEG795

Plasmid

#40122

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2) (mex-5 Nematode)

Use

Gateway donor vector

Mutation

Exon 2 recoded to be RNAi-resistant

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEG628

Plasmid

#40120

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)

Use

Gateway destination vector

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

contains aa1-244, Exon 2 recoded to be RNAi-resis…

Promoter

4.4 kb mex-5 promoter fragment

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
pFM037

Plasmid

#40140

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R163A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pEG196

Plasmid

#40074

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (mex-5 Nematode)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
MXW-PGK-c-let-7-LP4B-IRES-EGFP

Plasmid

#37647

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP4 - AATATTACCA=>AATAAAACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP4A-IRES-EGFP

Plasmid

#37646

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP4 - AATATTACCA=>AATAAAACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP6-IRES-EGFP

Plasmid

#37645

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP6 - AATATTACCA=>AATATTACAT

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP5-IRES-EGFP

Plasmid

#37644

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP5 - AATATTACCA=>AATATTTACA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP4-IRES-EGFP

Plasmid

#37643

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP4 - AATATTACCA=>AATAAAACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP3-IRES-EGFP

Plasmid

#37642

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP3 - AATATTACCA=>AAATTTACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MXW-PGK-c-let-7-LP2-IRES-EGFP

Plasmid

#37641

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Insert

cel-let-7

Use

Retroviral

Expression

Mammalian

Mutation

LP2 - AATATTACCA=>TTTATTACCA

Promoter

mPGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge