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  16. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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  20. Burden Monitor lambda version

    Plasmid
    #66073
    Purpose
    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:
    Insert
    GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1721 - 1740 of 158104 results