-
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP935-Batf3-IRES2-FlpO-WPRE-neo
Plasmid#181771Purposetargeting vectorDepositorInsertBatf3 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX
Plasmid#208695PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-W105M-IRES-mCherry
Plasmid#68857PurposeExpresses Human CDK8 W105M MutantDepositorInsertCyclin-Dependent Kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204343PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR del52-FLAG
Plasmid#204523PurposeRetroviral expression of human CALR del52-FLAGDepositorInsertCALR del52-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationdel52PromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry
Plasmid#173030PurposeTo express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cellsDepositorInsertSARS-CoV-2 Nucleocapsid phosphoprotein
UseLentiviralTags3xHA and IRES-mcherryExpressionMammalianMutationPromoterGag (truncated)Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-DDdCas9VP192-T2A-EGFP-ires-puro
Plasmid#69534PurposeDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.DepositorInsertDD-dCas9VP192-T2A-EGFP
UseTagsC-term T2A-EGFP and DHFR Destabilised DomainExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN PSAM4 GlyR IRES EGFP
Plasmid#119742PurposeChemogenetic inhibitor expression plasmidDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry
Plasmid#122957PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expressionDepositorInsertNOTCH2NLB (NOTCH2NLB Human)
UseLentiviralTags6xmyc tagExpressionMutationPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSF-1Rwt-IRES-GFP
Plasmid#115523PurposeMammalian expression constructDepositorInserthuman CSF-1 receptor (CSF1R Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR ins5-FLAG
Plasmid#204524PurposeRetroviral expression of human CALR ins5-FLAGDepositorInsertCALR ins5-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationins5PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50456PurposeDouble floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoterDepositorInsertrM3D-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
H516 SONIC KrasG12D-IRES-luciferase donor
Plasmid#138178PurposeCRISPR SONIC: Kras G12D luciferase donor plasmid.DepositorInsertKrasG12D (Kras Mouse)
UseNhej donorTagsExpressionMutationPromoterAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast
Plasmid#213117PurposeExpression of a faster-running MinE mutant in mammalian cellsDepositorInsertMinE(L4E)-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationL4EPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR
Plasmid#204497PurposeExpresses rtTA and Neomycin resistance genes in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A
Plasmid#205760PurposeSingle-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transitionDepositorInsertYFP-SLBP(18-126)-IRES-H1-Skylan62A
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W
Plasmid#32522DepositorInsertdominant negative Rheb (D60K) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationD60K to make dominant negativePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TRE3G-BCCP-PupE-IRES-BFP
Plasmid#113405PurposeExpress inducible BCCP-PupE in mammalian cellDepositorInsertBCCP-PupE-IRES-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only