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RC(WW)-LgBit

Plasmid

#208931

Purpose

Recognition domain for in vitro MAPK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

WW domain of PIN1 (PIN1 Human)

Use

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDT-SuperFi-Cas9

Plasmid

#183630

Purpose

Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

Depositor

David Taylor

Article

Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

Insert

SuperFi-Cas9

Use

CRISPR

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

Promoter

T7

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Use

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSD117A

Plasmid

#218141

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40

Use

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBXPHM3-PCFT_NB

Plasmid

#165415

Purpose

Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT_NB (SLC46A1 Llama, Chicken)

Use

Tags

His10-MBP-3C protease site

Expression

Bacterial

Mutation

Promoter

arabinose

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Use

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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p300 KAT LIC 1M

Plasmid

#233587

Purpose

Bacterial expression of p300 KAT enzyme

Depositor

Scott Showalter

Article

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300 KAT (EP300 Human)

Use

Tags

6xHis MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-FL

Plasmid

#216515

Purpose

Expresses MBP-tagged full length hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2 FL

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-IDR

Plasmid

#216517

Purpose

Expresses MBP-tagged intrinsically disordered region of hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-IDR

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-225

Promoter

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAM90

Plasmid

#226682

Purpose

Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

Rpn5 (RPN5 Budding Yeast)
Rpn6 (RPN6 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)
Rpn9 (RPN9 Budding Yeast)
Rpn11 (RPN11 Budding Yeast)

Use

Tags

MBP-HRV

Expression

Bacterial

Mutation

S111TAG

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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