We narrowed to 2,124 results for: PAM

1. 3XFLAG-NSarQ27G-VMAn-FKBP

   Plasmid

   #70229

   Purpose

   Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (N-terminal portion)
   

   Tags

   3XFLAG, FK506- and rapamycin-binding protein (FKB…

   Expression

   Mammalian

   Mutation

   Changed Gln 27 to Gly

   Promoter

   CMV

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCB6-HFE-EGFP

   Plasmid

   #12104

   Depositor

   Pamela Bjorkman

   Article

   Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

   Insert

   HFE (HFE Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-NGFP-VMAn-FKBP

   Plasmid

   #70225

   Purpose

   Expresses N-terminal portion of GFP along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   eGFP
   

   Tags

   FK506- and rapamycin-binding protein (FKBP), Link…

   Expression

   Mammalian

   Mutation

   Changed Glu 125 to Ile, Deleted amino acids 129-2…

   Promoter

   CMV

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pSH1/M-FRBl2-E

   Plasmid

   #15289

   Depositor

   David Spencer

   Article

   Li et al Gene Ther. 2002 Feb . 9(4):233-44.

   Insert

   FRAP-rapamycin binding domain (MTOR Human)
   

   Tags

   HA epitope and Myristoylation-targeting domain c-…

   Expression

   Mammalian

   Mutation

   res. 2025-2113, T2098L

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. PD29

   Plasmid

   #164542

   Purpose

   Expresses Drd1-FLAG

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Dopamine receptor D1 (Drd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXD70LacZ2-Pdadh-LacZ

   Plasmid

   #191618

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lentiviral-FKBP-cpGFP-Nb39

   Plasmid

   #191498

   Purpose

   Rapamycin-dependent opioid sensor for opioid receptors (component 2)

   Depositor

   Wenjing Wang

   Article

   Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

   Insert

   FKBP-cpGFP-Nb39
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mVenus-RapR-PTPPest

   Plasmid

   #188657

   Purpose

   Rapamycin regulated PTPPest with mVenus tag

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   Protein Tyrosine Phosphatase Pest (PTPN12 )
   

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK506

   Plasmid

   #155382

   Purpose

   dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lentiviral-MOR-FRB

    Plasmid

    #191497

    Purpose

    Rapamycin-dependent opioid sensor for the mu opioid receptor (component 1)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    MOR-FRB
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV2-GCaMP5g-P2A-Vmn1r58

    Plasmid

    #133418

    Purpose

    Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lentiviral-KOR-FRB

    Plasmid

    #191496

    Purpose

    Rapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    KOR-FRB
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCB6HindIII-rat FcRn

    Plasmid

    #121671

    Purpose

    Expresses rat neonatal Fc receptor in mammalian cells

    Depositor

    Pamela Bjorkman

    Article

    Tesar et al Traffic. 2006 Sep;7(9):1127-42.

    Insert

    rat neonatal Fc receptor (Fcgrt Rat)
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Rap-MESA-20-M-Gal4

    Plasmid

    #84554

    Purpose

    MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    Rap-MESA-FRB-20-M-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRibo-Tt

    Plasmid

    #154124

    Purpose

    Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Inserts

    engineered bacterial rRNAs operon under control of pL promotor
    bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptac and pL

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-FRB(PLF)-HA no-pA

    Plasmid

    #82597

    Purpose

    3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Mutation

    K2095P, T2098L, W2101F

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPBpuro-LDR

    Plasmid

    #173857

    Purpose

    Encoding a part of the rapamycin-induced dimerization system.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    a Lyn11-targeted FRB (LDR)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only