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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,257 results for: PAM

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Showing: 1 - 20 of 2257 results

  2. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Expression
    Bacterial
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  6. pAM57

    Plasmid
    #227654
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, V80A
    Promoter
    T7
    Available Since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227654-plasmid-map-sequence-id-455272

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  14. pAM4787

    Plasmid
    #120088
    Purpose
    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    Use
    Other
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120088-plasmid-map-sequence-id-234398

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  17. Protein2PAM_NmeN4C.1

    Plasmid
    #250218
    Purpose
    Express Protein2PAM_NmeN4C.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4C.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 E954C/Q981G/F1020Y/A1021S/C1023L/H1024D/…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250218-plasmid-map-sequence-id-501771

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  18. Protein2PAM_NmeN4G.1

    Plasmid
    #250224
    Purpose
    Express Protein2PAM_NmeN4G.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 D957G/V979I/V980K/Q981A/Q989T/N996E/S100…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250224-plasmid-map-sequence-id-501770

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Showing: 1 - 20 of 2257 results