We narrowed to 159,258 results for: addgene

1. pTK229

   Plasmid

   #71256

   Purpose

   luciferase reposter with 229 bp of the HSV TK promoter

   Depositor

   Peter Cockerill

   Article

   Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

   Insert

   Thymidine Kinase promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   HSV Thymidine Kinase

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro-shNT

   Plasmid

   #109012

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMMP-MCS-IRES-mRFP

   Plasmid

   #36972

   Depositor

   Bill Sugden

   Article

   Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATG9A sgRNA

   Plasmid

   #207557

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-H2B-mScarlet-I

   Plasmid

   #182297

   Purpose

   Localization of mScarlet-I fluorescent protein to histone 2B

   Depositor

   Thierry Fournier, Severine Degrelle

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   mScarlet-I
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL1P4 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198048

   Purpose

   MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCP-EGFP-PiggyBac

   Plasmid

   #190003

   Purpose

   Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

   Depositor

   Tineke Lenstra

   Article

   Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

   Insert

   MCP-EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVET-tTR-KRAB

   Plasmid

   #11644

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hEF-1alpha, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE puro PPAR gamma 2

    Plasmid

    #8859

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. ULK1 sgRNA

    Plasmid

    #207559

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCAGCCAGGCCAGAAAGGTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ST-GFP

    Plasmid

    #162500

    Purpose

    Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. WIPI2 sgRNA

    Plasmid

    #207554

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CGCGCGCCCAGCCATGAACC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACUH

    Plasmid

    #58374

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Yuh-Nung Jan, Chun Han

    Article

    Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-PGK-CXCR4 (neo)

    Plasmid

    #192077

    Purpose

    Lentivirus for expression of CXCR4

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7TG

    Plasmid

    #24314

    Depositor

    Roel Nusse

    Article

    Brugmann et al Development. 2007 Sep . 134(18):3283-95.

    Insert

    7xTcf-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. VPS35 shRNA2

    Plasmid

    #163859

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. ERoxBFP

    Plasmid

    #68126

    Purpose

    mammalian expression of ER localized oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    oxBFP
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only