We narrowed to 6,931 results for: crispr cas9 plasmids

1. pDonor_hU6

   Plasmid

   #69312

   Purpose

   sgRNA scaffold and human U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   hU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAH233

   Plasmid

   #121144

   Purpose

   Expression of gRNA with BbsI placeholder for target cloning

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGE_En03

   Plasmid

   #71535

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Sumihare Noji

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

   Plasmid

   #117168

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTJK459

   Plasmid

   #138008

   Purpose

   Empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Tami Kingsbury

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   U-A flip and hairpin extension: cgtttAagagctaTGCT…

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC gRNA Shuttle

   Plasmid

   #47024

   Purpose

   Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Insert

   gRNA Shuttle
   

   Use

   CRISPR; Cas9

   Tags

   Expression

   Plant

   Mutation

   G to T cloning mutation at position 323

   Promoter

   Medicago truncatula U6.6

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBlu/gRNA

   Plasmid

   #59188

   Purpose

   Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Guide RNA Cassette
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6 (Arabidopis)

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSQT834

   Plasmid

   #53371

   Purpose

   Csy4 and Cas9 nuclease expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCY5h

    Plasmid

    #160295

    Purpose

    Yeast CRISPR plasmid targeting the hphMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCY5n

    Plasmid

    #160296

    Purpose

    Yeast CRISPR plasmid targeting the natMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCY5k

    Plasmid

    #160294

    Purpose

    Yeast CRISPR plasmid targeting the kanMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTF1.1.0-gDNA

    Plasmid

    #113797

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1

    Depositor

    Kevin White

    Insert

    MTF1 (MTF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZBTB9.1.0-gDNA

    Plasmid

    #112469

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9

    Depositor

    Kevin White

    Insert

    ZBTB9 (ZBTB9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZBTB5.1.0-gDNA

    Plasmid

    #112399

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB5

    Depositor

    Kevin White

    Insert

    ZBTB5 (ZBTB5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZNF436.1.0-gDNA

    Plasmid

    #113767

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF436

    Depositor

    Kevin White

    Insert

    ZNF436 (ZNF436 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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