We narrowed to 3,796 results for: golden gate

1. EC71174

   Plasmid

   #154067

   Purpose

   Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   HS Cre recombinase
   Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
   HYGROMYCIN Resistance Cassette
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Expression

   Bacterial and Plant

   Mutation

   The Arabidopsis thaliana U5 small nuclear ribonuc…

   Promoter

   35S promoter, Hordeum vulgare HSP 17 promoter use…

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a, U6

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJet1.2 ermB cassette

   Plasmid

   #117121

   Purpose

   Erythromycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Erythromycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJet1.2 kanR cassette

   Plasmid

   #117122

   Purpose

   Kanamycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Kanamycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. G555

   Plasmid

   #216483

   Purpose

   Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

   Depositor

   Christophe Danelon

   Insert

   plsB, plsC, cdsA, pssA
   

   Use

   Cell-free expression

   Promoter

   T7 for each gene

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV GG hUbC-dsRED

   Plasmid

   #84034

   Purpose

   Contains Golden Gate cassette to express up to four gRNAs and dsRED

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBL101gg

   Plasmid

   #199735

   Purpose

   Golden Gate recipient vector for transgene overexpression

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   sacB
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJPC259

   Plasmid

   #234531

   Purpose

   Cre/Lox 71/66 Recombinase switch; plant T-DNA

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   RUBY
   

   Expression

   Plant

   Mutation

   none

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPlantPOP

   Plasmid

   #241283

   Purpose

   Cloning gene libraries from oligonucleotide pools

   Depositor

   Sean Cutler

   Article

   Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

   Type

   Empty backbone

   Use

   Other

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_C'0000a

    Plasmid

    #127226

    Purpose

    Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_D0000a

    Plasmid

    #127230

    Purpose

    Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBR_attB(bxb)_ccdB_lox_v2

    Plasmid

    #198040

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGF007

    Plasmid

    #48847

    Purpose

    Provides a kanamycin resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pNOS::KanamycinR::tNOS
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJet1.2 cat cassette

    Plasmid

    #117119

    Purpose

    Chloramphenicol resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Chloramphenicol resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_SYCP3_nterm

    Plasmid

    #222524

    Purpose

    Cas9/sgRNA plasmid for targeting SYCP3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, SYCP3 sgRNA (SYCP3 Human, Synthetic)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJB1578

    Plasmid

    #218620

    Purpose

    Express Pex22, Pex6, Pex15, and Pex8

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex22
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGGZ003

    Plasmid

    #48869

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGZ001

    Plasmid

    #48868

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR8/GW:U6

    Plasmid

    #85981

    Purpose

    Golden Gate Level 0 cassette encoding the U6 promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    T. pseudonana U6 promoter
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Promoter

    none

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJPC210

    Plasmid

    #234532

    Purpose

    Int13 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Expression

    Plant

    Mutation

    none

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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