We narrowed to 3,583 results for: golden gate

1. pKDcas9 fis cerulean

   Plasmid

   #230942

   Purpose

   aTc inducible knockdown of Fis

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKDcas9 mcaS cerulean

   Plasmid

   #230944

   Purpose

   aTc inducible knockdown of mcaS

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDcas9 csgD cerulean

   Plasmid

   #230945

   Purpose

   aTc inducible knockdown of CsgD

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDcas9 flhDC cerulean

   Plasmid

   #230946

   Purpose

   aTc inducible knockdown of FlhDC

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKDcas9 FliZ cerulean

   Plasmid

   #230947

   Purpose

   aTc inducible knockdown of FliZ

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKDcas9 23 cerulean

   Plasmid

   #230949

   Purpose

   aTc inducible knockdown of Fis and HNS

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKDcas9 45 cerulean

   Plasmid

   #230951

   Purpose

   aTc inducible knockdown of mcaS and CsgD

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKDcas9 56 cerulean

   Plasmid

   #230952

   Purpose

   aTc inducible knockdown of CsgD and FlhDC

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDcas9 67 cerulean

   Plasmid

   #230953

   Purpose

   aTc inducible knockdown of FlhDC and FliZ

   Depositor

   Andrew Griffiths

   Article

   Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   additional tetO

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTE5415

    Plasmid

    #229030

    Purpose

    Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR

    Depositor

    Tobias Erb

    Article

    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

    Insert

    PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt
    

    Use

    Tags

    10x His-tag + Factor Xa recognition site

    Expression

    Bacterial

    Mutation

    Promoter

    PT7-lacO and T7 promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTE5444

    Plasmid

    #231064

    Purpose

    Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR

    Depositor

    Tobias Erb

    Article

    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

    Insert

    PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak
    

    Use

    Tags

    10x His-tag + Factor Xa recognition site

    Expression

    Bacterial

    Mutation

    Promoter

    PT7-lacO and T7 promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTE5448

    Plasmid

    #231065

    Purpose

    Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR

    Depositor

    Tobias Erb

    Article

    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

    Insert

    PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSakOk
    

    Use

    Tags

    10x His-tag + Factor Xa recognition site

    Expression

    Bacterial

    Mutation

    Promoter

    PT7-lacO and T7 promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTE5452

    Plasmid

    #231067

    Purpose

    Inactive tetO-10xHis-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-10xHis-p2 dead-T7 terminator, AmpR

    Depositor

    Tobias Erb

    Article

    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

    Insert

    PT7-tetO-B0035-10xHis-phi29 DNAP dead-T7term
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7-tetO

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEVA63_sgRNA-B0_M13ps

    Plasmid

    #231421

    Purpose

    M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJPC259

    Plasmid

    #234531

    Purpose

    Cre/Lox 71/66 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJPC060

    Plasmid

    #234535

    Purpose

    TRV2 expressing FLPe

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    FLPe recombinase
    

    Use

    Recombinase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJPC432

    Plasmid

    #234528

    Purpose

    FLP Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJPC263

    Plasmid

    #234530

    Purpose

    Cre/Lox P1 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSF503

    Plasmid

    #222587

    Purpose

    BB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locus

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    gsdA (gsdA )
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert,…

    Promoter

    tet-on

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSF8

    Plasmid

    #222592

    Purpose

    BB2 gsdA tet-on overexpression cassette

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    gsdA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert,…

    Promoter

    tet-on

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge