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pAV-U6+27-Tornado-F30-Squash-Broccoli

Plasmid

#177918

Purpose

Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Squash-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 2xdBroccoli

Plasmid

#169233

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-2xdBroccoli

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28c-F30-2xdBroccoli-47CAG

Plasmid

#237217

Purpose

Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

Depositor

Mingxu You

Article

Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

Insert

47 CAG trinucleotide repeats RNA

Expression

Bacterial

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

Plasmid

#216822

Purpose

Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-Broccoli#4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pET21-E. coli Initiation Factor 3 (IF3)

Plasmid

#31340

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

E. coli Initiation Factor 3

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
Psicheck2 COLIA1 full length 3'UTR

Plasmid

#26993

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

COL1A1 Full length 3' UTR (COL1A1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
E. coli 16S toehold switch sensor

Plasmid

#110698

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-Split-Broccoli-Toehold-Switch

Plasmid

#87316

Purpose

Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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aav-U6-broccoli-hsp68-mCherry-MCS-pA

Plasmid

#195411

Purpose

AAV9 based enhancer activity assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Type

Empty backbone

Use

AAV

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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