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Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M66L wgMDH
Plasmid#204247PurposeBacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM66LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M91Q wgMDH
Plasmid#204250PurposeBacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM91QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D87L wgMDH
Plasmid#204248PurposeBacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD87LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQmod3S-GG
Plasmid#191349PurposeClostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pA-RFP-rG2
Plasmid#188969PurposeIPTG inducible mCherry with sgRNADepositorInsertsmCherry
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - MPX
Plasmid#89619Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertMitocondrial Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfA
Plasmid#89616PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG inductionDepositorInsertElectron transport flavoprotein alpha subunit
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfB
Plasmid#89617PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG inductionDepositorInsertelectron-transfer-flavoprotein, alpha polypeptide
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TDR
Plasmid#89627PurposeExpresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG inductionDepositorInsertthiol dependent reductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NFDD
Plasmid#89624PurposeExpresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG inductionDepositorInsertNAD/FAD dependent dehydrogenase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-p50-ligase
Plasmid#87742PurposeIPTG-inducible expression of p50-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and human p50/NF-kB (amino acids 40-366)ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHT-6xR.ads-PxylA-DI
Plasmid#47386PurposeExpresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsads
dxs
idi
UseTags6xR and HisExpressionBacterialMutationPromoterPgrac and PxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
UseTagsMxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_AB
Plasmid#66004PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationQ58LPromoterT5Available sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126R wgMDH
Plasmid#204261PurposeBacterial expression of P126R wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126RPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127A wgMDH
Plasmid#204263PurposeBacterial expression of G127A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127P wgMDH
Plasmid#204265PurposeBacterial expression of G127P wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127PPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121F wgMDH
Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only