We narrowed to 20 results for: IPTG

...Resistance Key Features Genotype Use BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT...Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon...insoluble, or membrane proteins  T7 Express   IPTG-inducible expression of T7 RNAP from the genome;...vectors such as pQE or similar), inducible under IPTG (lac repressor on the pREP4 plasmid). Expression...promoters, which are 'inactive' until an inducer such as IPTG is added to the growth medium. Induction timing ...repressor, which is inducible by the addition of IPTG. T7 RNAP is then available to transcribe the gene...of expression exists even without the addition of IPTG. This is mostly a problem for toxic protein products...

...lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate, IPTG binds and inactivates...turning the bacterial colony blue. We should note that IPTG is also included in the medium to ensure transcription... this plate should be blue, indicating that your IPTG and x-gal are working as they should be. Don't...

... number of the plasmid by altering IPTG concentrations. Higher IPTG concentration leads to higher RepA...a trans-acting RepA protein under the control of IPTG. Because RepA is a plasmid replication initiator...copy number. When they increased concentrations of IPTG, a constitutive promoter upstream of GFP increased...

...3xPtac Lactose/IPTG Escherichia coli Cheryl Arrowsmith 26094 pET28a-LIC T7-lacO Lactose/IPTG Escherichia ... coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic Esposito 43796 pDawn FixK2...Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia coli Cheryl Arrowsmith 46886 pMSP3535...bacteria Gary Dunny 74064 pZ8-Ptac Ptac + LacI Lactose/IPTG Corinebacterium glutamicum Timothy Lu 17972 pSE100... Christopher Marx 122635 pPEPZ-Plac Plac Lactose/IPTG Streptococcus pneumoniae Jan-Willem Veening 188978...Escherichia coli Cynthia Collins 222348 pJMP3653 PabstBR IPTG Escherichia coli , Acinetobacter baumannii Jason...

...is induced prior to inoculation with IPTG. In the absence of IPTG after inoculation, no more fluorescent...

...the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression to work, students have...

...also controlled by lac operator. Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated...lac repressor (lacI or lacIq). Can be induced by IPTG or lactose. Leaky promoter with somewhat weak expression...

...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...

...lifted onto nitrocellulose membranes and exposed to IPTG in order to induce protein expression. Cells on ...containing an appropriate inducing agent (0.4 mM IPTG is a good start if you’re using a pET vector) and...

...your culture and inducing protein expression with IPTG results in in vivo biotinylation. The co-expressed...

...variants: pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein CAG: Generic...

...activated. In the presence of lactose or lactose analog IPTG, the lac repressor undergoes a conformational change...

...CMV promoter, forms blue colonies on LB/Amp/Xgal/IPTG plate pAdx-CMV-copGFP 73346 Expresses copGFP from...

... subtilis to the bacterium of interest following IPTG induction of conjugation genes and bi-parental mating...

...blue colony phenotype upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction...

... SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies, the authors...

...lac repressor (lacI or lacIq). Can be induced by IPTG or lactose araBAD Inducible by arabinose Promoter...lac repressor (lacI or lacIq). Can be induced by IPTG or lactose Eukaryotic Promoters Eukaryotic promoters...

...Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression systems...

..., an arabinose inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon...

...commonly used to control expression of Red include the IPTG-inducible lac promoter, the arabinose-inducible ...