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We narrowed to 16 results for: IPTG

Showing: 1 - 16 of 16 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...Resistance Key Features Genotype Use BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT...Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon...insoluble, or membrane proteins  T7 Express   IPTG-inducible expression of T7 RNAP from the genome;...vectors such as pQE or similar), inducible under IPTG (lac repressor on the pREP4 plasmid). Expression...promoters, which are 'inactive' until an inducer such as IPTG is added to the growth medium. Induction timing ...repressor, which is inducible by the addition of IPTG. T7 RNAP is then available to transcribe the gene...of expression exists even without the addition of IPTG. This is mostly a problem for toxic protein products...

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate, IPTG binds and inactivates...turning the bacterial colony blue. We should note that IPTG is also included in the medium to ensure transcription... this plate should be blue, indicating that your IPTG and x-gal are working as they should be. Don't...

  3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ... number of the plasmid by altering IPTG concentrations. Higher IPTG concentration leads to higher RepA...a trans-acting RepA protein under the control of IPTG. Because RepA is a plasmid replication initiator...copy number. When they increased concentrations of IPTG, a constitutive promoter upstream of GFP increased...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...also controlled by lac operator. Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated...lac repressor (lacI or lacIq). Can be induced by IPTG or lactose. Leaky promoter with somewhat weak expression...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...lifted onto nitrocellulose membranes and exposed to IPTG in order to induce protein expression. Cells on ...containing an appropriate inducing agent (0.4 mM IPTG is a good start if you’re using a pET vector) and...

  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...activated. In the presence of lactose or lactose analog IPTG, the lac repressor undergoes a conformational change...

  13. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies, the authors...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression systems...
Showing: 1 - 16 of 16 results