...Resistance Key Features Genotype Use BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT...Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon...insoluble, or membrane proteins  T7 Express   IPTG-inducible expression of T7 RNAP from the genome;...vectors such as pQE or similar), inducible under IPTG (lac repressor on the pREP4 plasmid). Expression...promoters, which are 'inactive' until an inducer such as IPTG is added to the growth medium. Induction timing ...repressor, which is inducible by the addition of IPTG. T7 RNAP is then available to transcribe the gene...of expression exists even without the addition of IPTG. This is mostly a problem for toxic protein products...

...lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate, IPTG binds and inactivates...turning the bacterial colony blue. We should note that IPTG is also included in the medium to ensure transcription... this plate should be blue, indicating that your IPTG and x-gal are working as they should be. Don't...

... number of the plasmid by altering IPTG concentrations. Higher IPTG concentration leads to higher RepA...a trans-acting RepA protein under the control of IPTG. Because RepA is a plasmid replication initiator...copy number. When they increased concentrations of IPTG, a constitutive promoter upstream of GFP increased...

...is induced prior to inoculation with IPTG. In the absence of IPTG after inoculation, no more fluorescent...

...the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression to work, students have...

...also controlled by lac operator. Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated...lac repressor (lacI or lacIq). Can be induced by IPTG or lactose. Leaky promoter with somewhat weak expression...

...lifted onto nitrocellulose membranes and exposed to IPTG in order to induce protein expression. Cells on ...containing an appropriate inducing agent (0.4 mM IPTG is a good start if you’re using a pET vector) and...

...your culture and inducing protein expression with IPTG results in in vivo biotinylation. The co-expressed...

...activated. In the presence of lactose or lactose analog IPTG, the lac repressor undergoes a conformational change...

...variants: pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein CAG: Generic...

... subtilis to the bacterium of interest following IPTG induction of conjugation genes and bi-parental mating...

...blue colony phenotype upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction...

... SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies, the authors...

..., an arabinose inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon...

...Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression systems...

...commonly used to control expression of Red include the IPTG-inducible lac promoter, the arabinose-inducible ...