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We narrowed to 5,049 results for: golden gate

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Showing: 401 - 450 of 5049 results

  2. pGGG-TaBSS2-TaSEP1B6(int)

    Plasmid
    #242016
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242016-plasmid-map-sequence-id-504530

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  3. pGGG-TaBSS2-TaMOFB1(int)

    Plasmid
    #242015
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242015-plasmid-map-sequence-id-504508

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  4. pICSL47732_RFP

    Plasmid
    #245698
    Purpose
    Level 1 Position 1 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    245698-plasmid-map-sequence-id-501924

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  5. pICSL47791_RFP

    Plasmid
    #245704
    Purpose
    Level 1 Position 7 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    245704-plasmid-map-sequence-id-498370

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  6. pICSL47751_RFP

    Plasmid
    #245700
    Purpose
    Level 1 Position 3 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    245700-plasmid-map-sequence-id-498369

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  15. pICSL47742_RFP

    Plasmid
    #245699
    Purpose
    Level 1 Position 2 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245699-plasmid-map-sequence-id-496059

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  16. pICSL47822_RFP

    Plasmid
    #245707
    Purpose
    Level 1 Position 3 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245707-plasmid-map-sequence-id-496058

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  17. pICSL47841_RFP

    Plasmid
    #245709
    Purpose
    Level 1 Position 5 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245709-plasmid-map-sequence-id-496057

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  18. pICSL47861_RFP

    Plasmid
    #245711
    Purpose
    Level 1 Position 7 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245711-plasmid-map-sequence-id-496055

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  19. pICSL47831_RFP

    Plasmid
    #245708
    Purpose
    Level 1 Position 4 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245708-plasmid-map-sequence-id-496053

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  20. pICSL47852_RFP

    Plasmid
    #245710
    Purpose
    Level 1 Position 6 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245710-plasmid-map-sequence-id-496051

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  21. pICSL47811_RFP

    Plasmid
    #245706
    Purpose
    Level 1 Position 2 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245706-plasmid-map-sequence-id-496050

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  22. pICSL47802_RFP

    Plasmid
    #245705
    Purpose
    Level 1 Position 1 REVERSE acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245705-plasmid-map-sequence-id-496049

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  23. pICSL47781_RFP

    Plasmid
    #245703
    Purpose
    Level 1 Position 6 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245703-plasmid-map-sequence-id-496039

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  24. pICSL47772_RFP

    Plasmid
    #245702
    Purpose
    Level 1 Position 5 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245702-plasmid-map-sequence-id-496038

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  25. pICSL47761_RFP

    Plasmid
    #245701
    Purpose
    Level 1 Position 4 FORWARD acceptor with Amil RFP negative selection cassette GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245701-plasmid-map-sequence-id-496037

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  26. pKT3Ts-goldyTALEN

    Plasmid
    #80330
    Purpose
    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence
    Depositor
    Jeffrey Essner
    Article
    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi
    Type
    Empty backbone
    Use
    TALEN
    Expression
    Bacterial
    Promoter
    T3
    Available Since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80330-plasmid-map-sequence-id-156328

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  28. pNDGG010

    Plasmid
    #231315
    Purpose
    Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231315-plasmid-map-sequence-id-462987

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  29. pNDGG022

    Plasmid
    #231306
    Purpose
    Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231306-plasmid-map-sequence-id-462978

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  30. pNDGG009

    Plasmid
    #231314
    Purpose
    Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231314-plasmid-map-sequence-id-462970

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  33. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

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  34. pICSL47869

    Plasmid
    #245720
    Purpose
    Level 1 Position 7B REVERSE acceptor (NO end-linker required) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245720-plasmid-map-sequence-id-496452

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  35. pICSL47799

    Plasmid
    #245719
    Purpose
    Level 1 Position 7B FORWARD acceptor (NO end-linker required) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245719-plasmid-map-sequence-id-496048

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  36. pR2B5

    Plasmid
    #245486
    Purpose
    pL1-R2-pAtUBI-RUBY-tRBCS (Golden Gate Level 1 module with a transcription unit expressing RUBY from the AtUBI promoter with the tRBC terminator for R2 position in Level 2 vector)
    Depositor
    Beth Rowan
    Insert
    pAtUBI-RUBY-tRBCS
    Expression
    Plant
    Promoter
    Polyubiquitin 10 gene from Arabidopsis thaliana
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245486-plasmid-map-sequence-id-492313

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  37. pR2B6

    Plasmid
    #245487
    Purpose
    pL1-R2-pLsUBI-RUBY-tLsUBI (Golden Gate Level 1 module with a transcription unit expressing RUBY from the LsUBI promoter with the tLsUBI terminator for R2 position in Level 2 vector)
    Depositor
    Beth Rowan
    Insert
    pLsUBI-RUBY-tLSUBI
    Expression
    Plant
    Promoter
    Polyubiquitin 4 gene (LOC111919935) from lettuce
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245487-plasmid-map-sequence-id-492314

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  38. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

    Plasmid
    #50624
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Mrna production
    Promoter
    T7
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50624-plasmid-map-sequence-id-75688

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  39. pICSL47781-LC

    Plasmid
    #245717
    Purpose
    Level 1 Position 6 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    245717-plasmid-map-sequence-id-498371

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  40. pICSL47791-LC

    Plasmid
    #245718
    Purpose
    Level 1 Position 7 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245718-plasmid-map-sequence-id-496047

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  41. pICSL47772-LC

    Plasmid
    #245716
    Purpose
    Level 1 Position 5 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245716-plasmid-map-sequence-id-496045

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  42. pICSL47751-LC

    Plasmid
    #245714
    Purpose
    Level 1 Position 3 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245714-plasmid-map-sequence-id-496044

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  43. pICSL47732-LC

    Plasmid
    #245712
    Purpose
    Level 1 Position 1 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245712-plasmid-map-sequence-id-496042

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  44. pICSL47761-LC

    Plasmid
    #245715
    Purpose
    Level 1 Position 4 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245715-plasmid-map-sequence-id-496040

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  45. pICSL47742-LC

    Plasmid
    #245713
    Purpose
    Level 1 Position 2 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245713-plasmid-map-sequence-id-496035

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  50. pXZ13lacZalpha

    Plasmid
    #160229
    Purpose
    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160229-plasmid-map-sequence-id-314764

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Showing: 401 - 450 of 5049 results