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pFA6a-SRS-FLAG:hphMX4

Plasmid

#134445

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-S6:kanMX6

Plasmid

#134434

Purpose

PCR-based C-terminal S6 tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

S6

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-A1:kanMX6

Plasmid

#134426

Purpose

PCR-based C-terminal A1 tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-SRS-FLAG:natMX4

Plasmid

#134448

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-SRS-FLAG:HIS3MX6

Plasmid

#134447

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-S6-FLAG:HIS3MX6

Plasmid

#134439

Purpose

PCR-based C-terminal S6-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

S6-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-S6-FLAG:natMX4

Plasmid

#134440

Purpose

PCR-based C-terminal S6-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

S6-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-SRS-FLAG:kanMX6

Plasmid

#134446

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-A1-FLAG:kanMX6

Plasmid

#134430

Purpose

PCR-based C-terminal A1-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-A1-FLAG:HIS3MX6

Plasmid

#134431

Purpose

PCR-based C-terminal A1-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-S6-FLAG:hphMX4

Plasmid

#134437

Purpose

PCR-based C-terminal S6-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

S6-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCasso

Plasmid

#207530

Purpose

Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA

Use

CRISPR; Pseudomonas vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAblo

Plasmid

#207528

Purpose

Conditionally-replicating in Pseudomonas plasmid for adenine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sg

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sgRNA

Use

CRISPR; Pseudomonas vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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BE4

Plasmid

#100802

Purpose

Expresses BE4 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEd

Plasmid

#193845

Purpose

Express TadCBEd (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SaCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
eNme2-C TadCBEd

Plasmid

#193841

Purpose

Express TadCBEd (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadCBEd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9 TadCBEa

Plasmid

#193782

Purpose

Express TadCBEa (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa-SpCas9D10A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE2

Plasmid

#195368

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…

Tags

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge