We narrowed to 216 results for: YPet

1. pcDNA3-Mito-CaNAR2

   Plasmid

   #64731

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal 30 amino acids from DAKAP1,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTet-SNAP-HCA_sensor

   Plasmid

   #127182

   Purpose

   Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
   

   Use

   Tags

   HisTag and StrepTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTet-p52-HDM2_sensor

   Plasmid

   #127184

   Purpose

   Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
   

   Use

   Tags

   HisTag and StrepTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PI(LM)-Rac1(WT)dc1.FLARE

   Plasmid

   #87590

   Purpose

   PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   PBD
   Rac1
   

   Use

   Tags

   YPet and dTurq

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b(+)-SNAP-HCA_sensor

   Plasmid

   #127181

   Purpose

   Bacterial expression of the fusion protein used for sulfa-drugs sensing

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
   

   Use

   Tags

   HisTag and StrepTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PI(DM)-Rac1(WT)dc1.FLARE

   Plasmid

   #87589

   Purpose

   PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   PBD
   Rac1
   

   Use

   Tags

   YPet and dTurq

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBbsr2-TSen

   Plasmid

   #209916

   Purpose

   A FRET biosensor for ADAM17 activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TSen
   

   Use

   Tags

   ECFP and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-FKBP-E2

   Plasmid

   #175243

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-TORCAR

   Plasmid

   #64927

   Purpose

   FRET-based biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HP35 tension sensor

    Plasmid

    #101250

    Purpose

    Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-HP35-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSIIbsr-Eevee-ROCK-NES

    Plasmid

    #209918

    Purpose

    A FRET biosensor for ROCK activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Eevee-ROCK-NES
    

    Use

    Lentiviral

    Tags

    ECFP and YPet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Rap1A FLARE

    Plasmid

    #134926

    Purpose

    Rap1A biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1A, mCerulean3, RaIGDS RBD, YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. F40 tension sensor

    Plasmid

    #101252

    Purpose

    Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-F40-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FL tension sensor module

    Plasmid

    #101170

    Purpose

    Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

    Depositor

    Carsten Grashoff

    Article

    Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

    Insert

    YPet(short)-FL-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. stabilized HP35 tension sensor module

    Plasmid

    #101251

    Purpose

    Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-HP35st-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPBbsr2-5102HRasCT(Picchu)

    Plasmid

    #209917

    Purpose

    A FRET biosensor for tyrosine kinase activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    5102HRasCT
    

    Use

    Tags

    YPet and nTurquoise-GL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tagSrc(WT)-mCer3 S208F/V224L

    Plasmid

    #179138

    Purpose

    mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA
    

    Use

    Tags

    mCerulean3 (S208F/V224L)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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