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Plasmid#201301PurposeD10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM partDepositorInsertD10: Promoter of TraesCS3D02G517600
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PROM_12
Plasmid#201303PurposeD12: Promoter of TraesCS4D02G131100 as Golden Gate compatible MoClo PROM partDepositorInsertD12: Promoter of TraesCS4D02G131100
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCLxR1
Plasmid#172567PurposeInducible gene expression, pCOLA origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAVR1
Plasmid#172563PurposeInducible gene expression, pACYC origin, VanRAM repressor, PVanCC promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKPY115
Plasmid#72669PurposeEncodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) ControlDepositorInsertThr251Gly-EcPheRS
UseTagsExpressionBacterialMutationThr251Gly in Alpha SubunitPromoterPBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINIT_cat
Plasmid#46858PurposeFX sequencing vector for subcloning, chloramphenicol markerDepositorTypeEmpty backboneUseSequencing vectorTagsExpressionMutationPromoternoneAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mCherry-FLAG:cat
Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2UGFP
Plasmid#1195DepositorInsertGFP w/ stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RFP-SKL
Plasmid#183726PurposeFor expression of RFP targeted to peroxisomesDepositorInsertmRFP1
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFP-SKL
Plasmid#183727PurposeFor exrpression of GFP targeted to peroxisomesDepositorInsertEGFP
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mCardinal-SKL
Plasmid#183728PurposeFor exrpression of mCardinal targeted to peroxisomesDepositorInsertmCardinal
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mNeptune2-SKL
Plasmid#183729PurposeFor exrpression of mNeptune2 targeted to peroxisomesDepositorInsertmNeptube2
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU1-C-cat
Plasmid#112791PurposeChloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)DepositorArticleInsertcat
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK293
Plasmid#73372PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanged histidine-59 to phenylalanine and histidi…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK698
Plasmid#71616PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tryptopha…PromoterT5Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK507
Plasmid#71597PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tryptopha…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV-sig-mCherry-PDGFRTM-WPRE
Plasmid#216668PurposeExpresses membrane-bound mCherry in mammalian cellsDepositorInsertmCherry
UseTagsPDGFR Transmembrane DomainExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only