Showing: 161 - 180 of 267 results
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Plasmid#167692PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRU206
Plasmid#167693PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCF820_U6-sgRNA-EF1a-mCherry2_lenti
Plasmid#138316PurposeLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFPDepositorInsertSpyCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID3S
Plasmid#188651PurposeExpresses SaAID3S and gRNA in mammalian cellsDepositorInsertsnSaCas9-AID3S--UGI
Sa-sgRNA
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterEF-1a and H1AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID2S
Plasmid#188652PurposeExpresses SaAID2S and gRNA in mammalian cellsDepositorInsertsAID2S-nSaCas9--UGI
Sa-sgRNA
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterEF-1a and H1AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. aureus scaffold CARGO array
Plasmid#191317PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn2
Plasmid#206963PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGATDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn
Plasmid#206965PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGTDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-pAG-TM
Plasmid#207318PurposeFunctional incorporation of protein AG (pAG) fusion protein into envelope of virionsDepositorInsertprotein AG
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pECTO
Plasmid#67683PurposepMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureusDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAS9counter
Plasmid#107192PurposeExpresses CAS9 and sgRNA for targeted counterselection in S. aureusDepositorInsertsCas9
sgRNA
UseTagsExpressionBacterialMutationPromoterprab17AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
UseTagsC terminal TEV GFP octa-His affinity tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertapmA (apmA Staphylococcus aureus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInsertermC (ermM Staphylococcus epidermis)
UseTags6xHisExpressionBacterialMutationPromoterPlacAvailabilityAcademic Institutions and Nonprofits only -
pLQ-KO-tgt50
Plasmid#126578PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-Pspac-donor-tgt50
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
LacD_WT_pET21a
Plasmid#29785DepositorInsertTagatose 1,6-diphosphate aldolase
UseTagsHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 161 - 180 of 267 results