Addgene

We narrowed to 305 results for: staphylococcus

Showing: 161 - 180 of 305 results

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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseB-6xHis

Plasmid

#214809

Purpose

Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease B

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0012

Plasmid

#117533

Purpose

Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

eSaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

R499A,Q500K,R654A,G655R

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a Halotag-CNA35

Plasmid

#131128

Purpose

Halotag-CNA35 peptide for Collagen labelling

Depositor

Samantha Stehbens

Article

Ju et al bioRxiv 2022

Insert

Halotag-CNA35

Use

Tags

Halotag

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-pAG-TM

Plasmid

#207318

Purpose

Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

protein AG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRU55

Plasmid

#167684

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn2

Plasmid

#206963

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRU206

Plasmid

#167693

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU322

Plasmid

#167683

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Use

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRU61

Plasmid

#167692

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID3S

Plasmid

#188651

Purpose

Expresses SaAID3S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

nSaCas9-AID3S--UGI
Sa-sgRNA

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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