We narrowed to 18 results for: staphylococcus

1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ... Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria ... useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...

2. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...size. From the pool of shorter orthologs, only Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...

3. What's New in CRISPR - Spring 2019

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   ...developed another AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome...

4. Components of CRISPR/Cas9

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   ...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  ...

5. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

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   ...off-target effects Modified PAM specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered...

6. Hot Plasmids and Viral Preps - May 2021

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   ...gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and ...

7. Antibodies 101: Immunoprecipitation

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   ..., Hjelm K, Sjöquist J (1972) Protein a from Staphylococcus aureus. Its isolation by affinity chromatography...

8. Lab Safety for Biosafety Levels One and Two

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   Protocol

   ... with pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae . BSL-2 includes ...

9. Microbiology Resources

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   ... Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus...

10. CRISPR History and Development for Genome Engineering

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    ...Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and ...Zhang F. 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature . 520(7546):186-91. PMID...

11. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...has fused LSD1 to the smaller nuclease-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid ...

12. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ...Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep 6: . https://doi.org/10.1038...

13. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...Sharp PA, Zhang F. In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 9;520(7546);186...

14. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...lymph nodes limits local humoral response to Staphylococcus aureus." PLoS Pathog 11.4 (2015): e1004827....

15. Zhang Lab CRISPR Page

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    ...Oct 19. PubMed . In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott ...

16. CRISPR Guide

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    ... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ...

17. CRISPR Guide

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    Guide

    ... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ...

18. Adeno-associated virus (AAV) Guide

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    Guide

    ...smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng Zhang lab has developed plasmids...