We narrowed to 19 results for: staphylococcus
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TypeBlog Post... Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria ... useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...
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Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...size. From the pool of shorter orthologs, only Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org... -
What's New in CRISPR - Spring 2019
TypeBlog Post...developed another AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome... -
Components of CRISPR/Cas9
TypeBlog Post...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) ... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and ... -
Antibodies 101: Immunoprecipitation
TypeBlog Post..., Hjelm K, Sjöquist J (1972) Protein a from Staphylococcus aureus. Its isolation by affinity chromatography... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol... with pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae . BSL-2 includes ... -
AAV Molecular Tools
TypeCollection...Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6 Zhang Labeling Tools These... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...has fused LSD1 to the smaller nuclease-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid ... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep 6: . https://doi.org/10.1038... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Sharp PA, Zhang F. In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 9;520(7546);186... -
Microbiology Resources
TypeCollection... Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus... -
CRISPR History and Development for Genome Engineering
TypeCollection...Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and ...Zhang F. 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature . 520(7546):186-91. PMID... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...lymph nodes limits local humoral response to Staphylococcus aureus." PLoS Pathog 11.4 (2015): e1004827.... -
Bacterial Expression Systems
TypeCollection...68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose... -
Zhang Lab CRISPR Page
TypeCollection...Oct 19. PubMed . In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott ... -
CRISPR Guide
TypeCollection... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ... -
CRISPR Guide
TypeGuide... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ... -
Adeno-associated virus (AAV) Guide
TypeGuide...smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng Zhang lab has developed plasmids...